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			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

577801 Sigma-AldrichAnti-Tau Mouse mAb (TAU-5)

This Anti-Tau Mouse mAb (TAU-5) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Tau.

Anti-Tau Mouse mAb (TAU-5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet
Citations

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
H, M, R, Sh	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the ~45-68 kDa Tau protein.
Catalogue Number	577801
Brand Family	Calbiochem®
Application Data	Detection of human tau by immunoblotting. Samples: Whole cell lysate from SH-SY5Y neuronal cells. Primary antibody: Anti-Tau Mouse mAb (TAU-5) (Cat No. 577801) (1:1000). Detection: chemiluminescence.

References
References	Papasozomenos, S.C. and Shanavas, A., 2002. Proc. Natl. Acad. Sci. USA 99, 1140. Rapoport, M., eta al. 2002. Proc. Natl. Acad. Sci. USA 99, 6364.

Product Information
Form	Liquid
Formulation	In PBS.
Preservative	None
Quality Level	MQ100

Applications
Key Applications	Frozen Sections Immunoblotting (Western Blotting) Immunoprecipitation Paraffin Sections
Application Notes	Frozen Sections (1-2 µg/ml) Immunoblotting (1 µg/ml) Immunoprecipitation (10 µg per 200-500 µg cell lysate) Paraffin Sections (1-2 µg/ml, heat pre-treatment required)
Application Comments	When used for formalin/paraffin embedded sections, staining is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min prior to antibody incubation. Alternate splicing of tau mRNA and differential phosphorylation contribute to the heterogeneity of tau. Does not cross-react with other MAPS of tubulin. Recognizes both native and phosphorylated forms of tau. Variables associated with assay conditions will dictate proper working dilution.

Biological Information
Immunogen	purified bovine microtubule-associated proteins
Immunogen	Bovine
Epitope	within the central region
Clone	TAU-5
Host	Mouse
Isotype	IgG₁
Species Reactivity	Human Mouse Rat Sheep
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	≤ -70°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-70°C). Do not store diluted antibody without carrier protein if the concentration is <50 µg/ml.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
577801-100UGCN	04055977265910

Documentation

Anti-Tau Mouse mAb (TAU-5) MSDS

职位
物料安全数据表 (MSDS)

Anti-Tau Mouse mAb (TAU-5) 分析证书

标题	批号
577801	输入批号

参考

参考信息概述
Papasozomenos, S.C. and Shanavas, A., 2002. Proc. Natl. Acad. Sci. USA 99, 1140. Rapoport, M., eta al. 2002. Proc. Natl. Acad. Sci. USA 99, 6364.

引用

标题

Hui, L., et al. 2012. Life Sci. 91, 1159.

Hui, L., et al. 2012. ASN Neuro. 4, 243.

Chen, X., et al. 2013. Neurobiol Aging 34, 2370.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	03-May-2010 JSW
Application	Frozen Sections (1-2 µg/ml) Immunoblotting (1 µg/ml) Immunoprecipitation (10 µg per 200-500 µg cell lysate) Paraffin Sections (1-2 µg/ml, heat pre-treatment required)
Application Data	Detection of human tau by immunoblotting. Samples: Whole cell lysate from SH-SY5Y neuronal cells. Primary antibody: Anti-Tau Mouse mAb (TAU-5) (Cat No. 577801) (1:1000). Detection: chemiluminescence.
Description	Protein G purified mouse monoclonal antibody. Recognizes the ~45-68 kDa phosphorylated and unphosphorylated forms of tau.
Background	Tau is a neuronal microtubule-associated protein found predominantly in axons. Tau promotes tubulin polymerization and stabilizes microtubules, and it links certain signaling pathways to the cytoskeleton. Hyperphosphorylated tau is the major component of paired helical filaments, the building block of neurofibrillary lesions in Alzheimer’s disease. Hyperphosphorylation impairs the microtubule-binding function of tau, resulting in the destabilization of microtubules, and ultimately leading to degeneration of the affected neurons.
Host	Mouse
Immunogen species	Bovine
Immunogen	purified bovine microtubule-associated proteins
Epitope	within the central region
Clone	TAU-5
Isotype	IgG₁
Species	human, mouse, rat, sheep
Form	Liquid
Formulation	In PBS.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	None
Comments	When used for formalin/paraffin embedded sections, staining is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min prior to antibody incubation. Alternate splicing of tau mRNA and differential phosphorylation contribute to the heterogeneity of tau. Does not cross-react with other MAPS of tubulin. Recognizes both native and phosphorylated forms of tau. Variables associated with assay conditions will dictate proper working dilution.
Storage	Avoid freeze/thaw ≤ -70°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-70°C). Do not store diluted antibody without carrier protein if the concentration is <50 µg/ml.
Toxicity	Standard Handling
References	Papasozomenos, S.C. and Shanavas, A., 2002. Proc. Natl. Acad. Sci. USA 99, 1140. Rapoport, M., eta al. 2002. Proc. Natl. Acad. Sci. USA 99, 6364.
Citation	Hui, L., et al. 2012. Life Sci. 91, 1159. Hui, L., et al. 2012. ASN Neuro. 4, 243. Chen, X., et al. 2013. Neurobiol Aging 34, 2370. .

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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