Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Detection of rat TUBB2A by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-TUBB2A Mouse mAb (2B2) (Cat. No. CB1052) (1-5 µg/ml). Detection: chemiluminescence.
Detection of mouse TUBB2A by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Primary antibody: Anti-TUBB2A Mouse mAb (2B2) (Cat. No. CB1052) (1-5 µg/ml). Detection: chemiluminescence.
Detection of human TUBB2A by immunoblotting. Sample: Whole cell lysate from Jurkat cells. Primary antibody: Anti-TUBB2A Mouse mAb (2B2) (Cat. No. CB1052) (1-5 µg/ml). Detection: chemiluminescence.
Detection of human TUBB2A by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human spleen tissue. Primary antibody: Anti-TUBB2A Mouse mAb (2B2) (Cat. No. CB1052) (1.5 µg/ml). Detection: colorimetric (DAB).
Description
Purified mouse monoclonal antibody. Recognizes the ~48-50 kDa TUBB2A protein.
Background
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the β-chain and one at a non-exchangeable site on the α-chain.
Host
Mouse
Immunogen
Full-length, recombinant, human TUBB2A (aa 1-446), expressed as a GST fusion protein
Clone
2B2
Isotype
IgG2a
Species
human, mouse, rat
Positive control
PC-12 cells, Raw 264.7 cells, Jurkat cells, NIH3T3 cells, Human spleen tissue
Negative control
293T cells
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).