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MABC985 Anti-TRAF4 Antibody, clone 5MLN-2H1

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MABC985
100 µL  
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      概述

      重要规格表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IHC, ICCMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABC985
      DescriptionAnti-TRAF4 Antibody, clone 5MLN-2H1
      Alternate Names
      • TNF receptor-associated factor 4
      • Cysteine-rich domain associated with RING and Traf domains protein 1
      • Metastatic lymph node gene 62 protein
      • MLN 62
      • RING finger protein 83
      Background InformationTNF receptor-associated factor 4 (TRAF4), also known as Cysteine-rich domain associated with RING and Traf domains protein 1 (CART1), Metastatic lymph node gene 62 protein (MLN 62), and RING finger protein 83 (RNF83), is an adapter protein and signal transducer that links members of the TNF family to different signaling pathways. As such TRAF4 plays a role in the activation of the NF-kappa- and JNK signaling as well as a player in apoptosis and cell survival pathways. In development, TRAF4 is necessary for proper skeleton development and lung development. TRAF4 is localized in the perinuclear region of the cell as well as other locations including cell junctions where it is associated with various cytoskeletal elements. TRAF4 is expressed in the epithelial cells of thymus, respiratory tract, salivary gland, and esophagus. TRAF4 expression is regulated via the ubiquitin proteasome system and is a polyubiquitin target protein.
      Product Information
      FormatAscites
      PresentationMouse monoclonal IgG1 ascites without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-TRAF4 Antibody, clone 5MLN-2H1 is validated for use in Western Blotting and Immunohistochemistry and Immunocytochemistry for the detection of TRAF4.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunocytochemistry
      Application NotesImunnohistochemistry Analysis: A representative lot of this antibody detected TRAF4 in multiple human cancer tissues (Camilleri-Broet. S., et al. (2007) Oncogene. 26:142-147).

      Imunnohistochemistry Analysis: A representative lot of this antibody detected TRAF4 in Breast cancer tissue (Kedinger, V., et. al. (2008) PLoS ONE. 3(10):e3518).

      Immunocytochemistry Analysis: A representative lot of this antibody detected TRAF4 in MCF-10A cells and tranfected HeLa cells (Kedinger, V., et. al. (2008) PLoS ONE. 3(10):e3518).
      Biological Information
      ImmunogenLinear peptide corresponding to Human TRAF4.
      Cloneclone 5MLN-2H1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityThis antibody detects TRAF4 and is cited in publications as both clone 5MLN-2H1 and clone 2H1.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Predicted to react with Rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TRAF4
      • CART1
      • MLN62
      • RNF83
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~53 kDa observed
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: A 1:1000 dilution of this antibody detected TRAF4 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      产品目录编号 GTIN
      MABC985 04055977052992