Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Immunoblotting (1:2000, see comments) Immunoprecipitation (1:100, see comments)
Application Comments
This antibody has been used to detect endogenous TORC2 by immunoblotting. For immunoprecipitation, 4 µl antibody in a total volume of 400 µl has been tested. May also detect a protein at ~60 kDa, which may be a degradation product of TORC2. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
a recombinant protein consisting of amino acids 454-607 of mouse TORC2 fused to GST
Immunogen
Human
Host
Rabbit
Isotype
IgG
Species Reactivity
Human
Mouse
Rat
Antibody Type
Polyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Standard Handling
Storage
+2°C to +8°C
Do not freeze
Ok to freeze
Special Instructions
For long-term storage, aliquot and freeze (-20°C). In the case of storage at -20°C, avoid freeze/thaw cycles.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
19-October-2007 RFH
Synonyms
Anti-Transducer of regulated cAMP response element-binding protein (CREB) 2, Anti-TORC2
Application
Immunoblotting (1:2000, see comments) Immunoprecipitation (1:100, see comments)
Application Data
Detection of rat TORC2 by immunoblotting. Sample: Whole cell lysate from primary rat hepatocytes. Primary antibody: Anti-TORC2 (454-607) Rabbit pAb (Cat. No. ST1099) (1:2000). Detection: chemiluminescence.
Description
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~79-85 kDa TORC2 protein.
Background
TORCs (Transducers Of Regulated cAMP Response Element-Binding (CREB) protein) are members of a conserved family of co-activators that enhance CRE-dependent transcription by a phosphorylation-independent interaction with the bZIP DNA binding/dimerization domain of CREB. Studies suggest that TORC phosphorylation and nuclear/cytoplasmic shuttling play an important role in the regulation of gluconeogenesis by cAMP.
Host
Rabbit
Immunogen species
Human
Immunogen
a recombinant protein consisting of amino acids 454-607 of mouse TORC2 fused to GST
Isotype
IgG
Species
human, mouse, rat
Positive control
Primary rat hepatocytes, HepG2, NIH3T3, Min6, and FAO cells
Form
Liquid
Formulation
Undiluted serum.
Preservative
None
Comments
This antibody has been used to detect endogenous TORC2 by immunoblotting. For immunoprecipitation, 4 µl antibody in a total volume of 400 µl has been tested. May also detect a protein at ~60 kDa, which may be a degradation product of TORC2. Antibody should be titrated for optimal results in individual systems.
Storage
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
For long-term storage, aliquot and freeze (-20°C). In the case of storage at -20°C, avoid freeze/thaw cycles.
