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This Anti-TNF-α Rabbit pAb is validated for use in ELISA, Frozen Sections, Immunoblotting, Neutralization Studies, Paraffin Sections, Radioimmunoassay for the detection of TNF-α.
More>>This Anti-TNF-α Rabbit pAb is validated for use in ELISA, Frozen Sections, Immunoblotting, Neutralization Studies, Paraffin Sections, Radioimmunoassay for the detection of TNF-α. Less<<
Anti-TNF-α Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
ELISA (see comments) Frozen Sections (see comments) Immunoblotting (see comments) Neutralization Studies (see comments) Paraffin Sections (see comments) Radioimmunoassay (see comments)
Application Comments
Reacts specifically with natural and recombinant human TNF-α. An approximately 20 µg/ml solution of this antibody has been shown to neutralize 100% of the activity of 0.5 ng/ml recombinant human TNF-α, as measured in the standard L929 cell bioassay. This antibody has also been reported to work for ELISA, frozen sections, immunoblotting, paraffin sections, radioimmunoassay. Endotoxin: ≤5 EU/mg protein as determined by the Biowhittaker Endotect LAL assay. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
recombinant, human TNF-α
Immunogen
Human
Host
Rabbit
Isotype
IgG
Species Reactivity
Human
Mouse
Antibody Type
Polyclonal Antibody
Concentration Label
Please refer to vial label for lot-specific concentration
Physicochemical Information
Contaminants
Endotoxins: ≤1 EU/mg
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Standard Handling
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
07-August-2009 JSW
Synonyms
Anti-Tumor Necrosis Factor-α
Application
ELISA (see comments) Frozen Sections (see comments) Immunoblotting (see comments) Neutralization Studies (see comments) Paraffin Sections (see comments) Radioimmunoassay (see comments)
Description
Rabbit polyclonal antibody purified by ammonium sulfate precipitation. Recognizes recombinant and monocyte-derived TNF-α.
Background
Human Tumor Necrosis Factor α (TNF-α, cachectin) is a cytokine, produced by variety of cell types, that is involved in mediation of the inflammatory response, resistance to infection, and resistance to tumor growth. Human and mouse TNF-α are approximately 79% homologous at the amino acid level and exhibit cross-species reactivity. This antibody recognizes both recombinant and natural human TNF-α and exhibits some (~30%) cross-reactivity with mouse TNF-α. It exhibits only minor (<1%) cross-reactivity with human TNF-β (lymphotoxin).
Host
Rabbit
Immunogen species
Human
Immunogen
recombinant, human TNF-α
Isotype
IgG
Species
human, mouse (weakly)
Positive control
L929 cells
Form
Liquid
Formulation
In PBS.
Concentration Label
Please refer to vial label for lot-specific concentration
Contaminants
Endotoxins: ≤1 EU/mg
Unit definition
1 EU corresponds to 0.1 ng endotoxin (EC-6/USP Standard)
Preservative
None
Comments
Reacts specifically with natural and recombinant human TNF-α. An approximately 20 µg/ml solution of this antibody has been shown to neutralize 100% of the activity of 0.5 ng/ml recombinant human TNF-α, as measured in the standard L929 cell bioassay. This antibody has also been reported to work for ELISA, frozen sections, immunoblotting, paraffin sections, radioimmunoassay. Endotoxin: ≤5 EU/mg protein as determined by the Biowhittaker Endotect LAL assay. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
