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			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

IM32 Sigma-AldrichAnti-TIMP-1 (Ab-1) Mouse mAb (7-6C1)

This Anti-TIMP-1 (Ab-1) Mouse mAb (7-6C1) is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of TIMP-1 (Ab-1).

Anti-TIMP-1 (Ab-1) Mouse mAb (7-6C1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

同义词: Anti-Tissue Inhibitor of Metalloproteinase-1

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
B, H, R	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the ~28.5 kDa TIMP-1 protein. Also recognizes MMP/TIMP-1 complexes.
Catalogue Number	IM32
Brand Family	Calbiochem®
Synonyms	Anti-Tissue Inhibitor of Metalloproteinase-1
Application Data	Detection of TIMP-1 by immunoblotting. Sample: Whole cell lysate from CHO cells. Primary antibody: Anti-TIMP-1 (Ab-1) Mouse mAb (7-6C1) (Cat. No. IM32) (1 µg/ml). Detection: chemiluminescence.

References
References	Fujimoto, N. and Iwata, K., 1994. Connective Tissue 26, 237. Cottam, D. W. and Rees, R. C., 1993. Intl. J. Oncol. 2, 861. Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434. Woessner, J. F., 1991. FASEB J. 5, 2145. Liotta, L. A. and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology ed. M. M. Gottesman. Vol. 99-106.

Product Information
Declaration	Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
Form	Liquid
Formulation	In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
Positive control	rTIMP-1 Protein (Cat. Nos. PF019 or PF020)
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Application References	Immunoblotting Kodama, S., et al. 1990. J. Immunol. Meth. 127, 103. Imunohistochemistry Okada, Y., et al. 1990. Virchows Arch. B Cell Pathol. 59, 305. Paraffin Sections Romanic, A.M., et al. 1998. Stroke 29, 1020. Immunocytochemistry Zhao, W.Q., et al. 1998. J. Cell Sci. 111, 1147. Original Clone Kodama, S., et al. 1987. Collagen Rel. Res. 7, 341.
Key Applications	Immunoblotting (Western Blotting) Immunocytochemistry Paraffin Sections
Application Notes	Immunoblotting (1 µg/ml, see application references) Immunocytochemistry (see application references) Paraffin Sections (25 µg/ml, see application references, see comments)
Application Comments	Also recognizes TIMP-1/MMP complexes.This antibody has been used to stain paraffin sections fixed with PLP (perioddate-lysine-4% paraformaldehyde) and 10% formaldehyde (using 25 µg/ml antibody). Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	purified, bovine dental pulp TIMP-1
Immunogen	Bovine
Clone	7-6C1
Host	Mouse
Isotype	IgG₁
Species Reactivity	Bovine Human Rat
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
IM32-100UGCN	07790788053765

Documentation

Anti-TIMP-1 (Ab-1) Mouse mAb (7-6C1) MSDS

职位
物料安全数据表 (MSDS)

Anti-TIMP-1 (Ab-1) Mouse mAb (7-6C1) 分析证书

标题	批号
IM32	输入批号

参考

参考信息概述
Fujimoto, N. and Iwata, K., 1994. Connective Tissue 26, 237. Cottam, D. W. and Rees, R. C., 1993. Intl. J. Oncol. 2, 861. Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434. Woessner, J. F., 1991. FASEB J. 5, 2145. Liotta, L. A. and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology ed. M. M. Gottesman. Vol. 99-106.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	27-August-2007 RFH
Synonyms	Anti-Tissue Inhibitor of Metalloproteinase-1
Application	Immunoblotting (1 µg/ml, see application references) Immunocytochemistry (see application references) Paraffin Sections (25 µg/ml, see application references, see comments)
Application Data	Detection of TIMP-1 by immunoblotting. Sample: Whole cell lysate from CHO cells. Primary antibody: Anti-TIMP-1 (Ab-1) Mouse mAb (7-6C1) (Cat. No. IM32) (1 µg/ml). Detection: chemiluminescence.
Description	Purified mouse monoclonal antibody (see application references). Recognizes the ~28 kDa TIMP-1 protein.
Background	Matrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMPs share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an approximately 10 kDa segment from the N-terminus and their activity is regulated by endogenous inhibitors. These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis. The activation and activity of MMPs are regulated by a family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP). TIMP-1 (also called EPA, Fibroblast collagenase inhibitor or collagenase inhibitor) is a 28 kDa glycoprotein that is expressed by a variety of cell types. It forms a non-covalent, stoichiometric complex with both latent and active MMPs. TIMP-1 preferentially binds and inhibits MMP-9. TIMPs are capable of altering the metastatic potential of cancer cells and have been shown to inhibit invasion and metastasis in animal models.
Host	Mouse
Immunogen species	Bovine
Immunogen	purified, bovine dental pulp TIMP-1
Clone	7-6C1
Isotype	IgG₁
Species	bovine, human, not mouse, rat
Positive control	rTIMP-1 Protein (Cat. Nos. PF019 or PF020)
Form	Liquid
Formulation	In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	Also recognizes TIMP-1/MMP complexes.This antibody has been used to stain paraffin sections fixed with PLP (perioddate-lysine-4% paraformaldehyde) and 10% formaldehyde (using 25 µg/ml antibody). Antibody should be titrated for optimal results in individual systems.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Standard Handling
References	Fujimoto, N. and Iwata, K., 1994. Connective Tissue 26, 237. Cottam, D. W. and Rees, R. C., 1993. Intl. J. Oncol. 2, 861. Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434. Woessner, J. F., 1991. FASEB J. 5, 2145. Liotta, L. A. and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology ed. M. M. Gottesman. Vol. 99-106.
Application references	Immunoblotting Kodama, S., et al. 1990. J. Immunol. Meth. 127, 103. Imunohistochemistry Okada, Y., et al. 1990. Virchows Arch. B Cell Pathol. 59, 305. Paraffin Sections Romanic, A.M., et al. 1998. Stroke 29, 1020. Immunocytochemistry Zhao, W.Q., et al. 1998. J. Cell Sci. 111, 1147. Original Clone Kodama, S., et al. 1987. Collagen Rel. Res. 7, 341.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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