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MABE1042 Anti-TDRD3 Antibody, clone 2E11

MABE1042
100 µg  
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      概述

      Replacement Information

      重要规格表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1042
      DescriptionAnti-TDRD3 Antibody, clone 2E11
      Alternate Names
      • TDRD3
      • Tudor domain-containing protein 3
      Background InformationTudor domain-containing protein 3 (UniProt Q9H7E2) is encoded by the TDRD3 gene in human (Entrez Gene ID 81550). TDRD3 belongs to an evolutionarily conserved family of 12 proteins (TDRD1-TDRD12) that contain one or more Tudor domains, which recognize symmetric dimethylarginine (sDMA) residues with the exception of TDRD3. Unlike other TDRD members, TDRD3 associates selectively with asymmetric dimethylarginine (aDMA or Rme2a) on histones and on RNA Polymerase II (RNAP II) C-terminal domain (CTD), but not with other methylarginine motifs. TDRD3 is reported to act as a positive regulator of c-MYC gene transcription by recruiting topoisomerase IIIB (TOP3B) to the c-MYC gene promoter region via H4R3me2a recognition. (PMID 23066109 & 24507716). Cat. No. MABE1042, Anti-TDRD3, clone 2E11, is a purified mouse monoclonal antibody (PMID 21172665) against an epitope within a region in the C-terminal half of all three human TDRD3 spliced isoforms (UniProt Q9H7E2). Clone 2E11 is suitable for Western blotting application (PMID 21172665 & 24507716).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ300
      Applications
      ApplicationAnti-TDRD3 Antibody, clone 2E11 is an antibody against TDRD3 for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected TDRD3 in HeLa and MCF7 cell lysate (Yang, Y., et al. (2014) Mol. Cell. 53(3):484-497;Yang, Y., et al. (2010) Mol. Cell. 40(6):1016-1023).
      Biological Information
      ImmunogenRecombinant protein corresponding to human TDRD3.
      Clone2E11
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TDRD3
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~80 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected TDRD3 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      产品目录编号 GTIN
      MABE1042 04055977165098

      Documentation

      Anti-TDRD3 Antibody, clone 2E11 分析证书

      标题批号
      Anti-TDRD3, clone 2E11 - 3250273 3250273
      Anti-TDRD3, clone 2E11 -Q2564191 Q2564191

      参考

      参考概述公共医疗ID
      Arginine methylation facilitates the recruitment of TOP3B to chromatin to prevent R loop accumulation.
      Yang, Y; McBride, KM; Hensley, S; Lu, Y; Chedin, F; Bedford, MT
      Molecular cell  53  484-97  2014

      显示摘要
      24507716 24507716
      TDRD3 is an effector molecule for arginine-methylated histone marks.
      Yang, Y; Lu, Y; Espejo, A; Wu, J; Xu, W; Liang, S; Bedford, MT
      Molecular cell  40  1016-23  2010

      显示摘要
      21172665 21172665

      小册子

      标题
      NPI Flyer- Epigenetics and Nuclear Function Feature

      技术信息

      标题
      White Paper: Further considerations of antibody validation and usage.