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Detection of human SMO by immunoblotting. Sample: Whole cell lysate from A431 cells. Primary antibody: Anti-SMO Mouse mAb (1D9) (Cat. No. ST1718) (1-5 µg/ml). Detection: chemiluminescence.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
10-November-2010 JLN
Synonyms
Anti-Smoothened
Application
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human SMO by immunoblotting. Sample: Whole cell lysate from A431 cells. Primary antibody: Anti-SMO Mouse mAb (1D9) (Cat. No. ST1718) (1-5 µg/ml). Detection: chemiluminescence.
Description
Purified mouse monoclonal antibody. Recognizes the ~75 kDa SMO protein.
Background
SMO is a G protein-coupled receptor that probably associates with the protein, Patched (PTCH), to transduce hedgehog signalling. Binding of sonic hedgehog (SHH) to its receptor, Patched, is thought to inhibit the interaction between SMO and Patched.
Host
Mouse
Immunogen
Full-length, recombinant, human SMO (aa 653-787) expressed as a GST fusion protein
Clone
1D9
Isotype
IgG2a
Species
human
Positive control
A431 cells
Form
liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
