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MABN1832 Sigma-AldrichAnti-SK2 (KCa2.2) Antibody, clone K78/29

Anti-SK2 (KCa2.2) Antibody, clone K78/29 is an antibody against SK2 for use in Western Blotting, Immunohistochemistry (Paraffin).

Anti-SK2 (KCa2.2) Antibody, clone K78/29 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters Gene Symbol: Kcnn2 UniProt Number: P70604

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
R, H, M	WB, IH(P)	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABN1832
Description	Anti-SK2 (KCa2.2) Antibody, clone K78/29
Alternate Names	Small conductance calcium-activated potassium channel protein 2 SK2 SKCa 2 SKCa2 KCa2.2
Background Information	Small conductance calcium-activated potassium channel protein 2 (UniProt P70604; also known as SK2, SKCa 2, SKCa2, KCa2.2) is encoded by the Kcnn2 gene (Gene ID 54262) in rat species. Small conductance calcium-activated potassium channels mediate cell membrane repolarization following intracellular Ca2+ increase induced by various stimuli, thereby restoring an excited cell back to its resting state. SK2 is involved in afterhyperpolarization (AHP) that follows action potentials in vertebrate neurons. SK2 regulates neuronal excitability by contributing to the slow component of synaptic AHP. Ca2+ activates SK2 through the Ca2+ sensor calmodulin (CaM), which can bind up to four Ca2+ ions. The dynamic coupling between Ca2+ binding to CaM, CaM binding to SK, and the open probability of SK are all essential components of the SK2 activity.

References

Product Information
Format	Purified
Presentation	Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-SK2 (KCa2.2) Antibody, clone K78/29 is an antibody against SK2 for use in Western Blotting, Immunohistochemistry (Paraffin).
Key Applications	Western Blotting Immunohistochemistry (Paraffin)
Application Notes	Immunohistochemistry Analysis: An 1:50 dilution from a representative lot detected SK2 (KCa2.2) immunoreactivity in human cerebellum, human cerebral cortex, mouse brain, mouse skeletal muscle, rat kidney and rat testis tissue sections. Western Blotting Analysis: A representative lot detected the transiently expressed rat SK2 (KCa2.2), but not SK1 (KCa2.1), SK3 (KCa2.3), SK4 (KCa3.1, KCa4), or Kv2.1 constructs in transfected COS-7 cells (Courtesy of Professor. James S. Trimmer, UC Davis, CA, USA). Immunohistochemistry Analysis: A representative lot detected SK2 (KCa2.2) immunoreactivity in adult rat brain hippocampus sections (Courtesy of Professor. James S. Trimmer, UC Davis, CA, USA).

Biological Information
Immunogen	Linear peptide corresponding to a C-terminal cytoplasmic domain sequence of rat SK2 (KCa2.2).
Epitope	C-terminal cytomplasmic region.
Clone	K78/29
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Specificity	Clone K78/29 detects endogenous KCa2.2 in rat brain membrane extracts, as well as the transiently expressed rat SK2 (KCa2.2), but not SK1 (KCa2.1), SK3 (KCa2.3), SK4 (KCa3.1, KCa4), or Kv2.1 constructs in transfected COS-7 cells. Exprected to react with both spliced isoforms of human KCa2.2 reported by UniProt (Q9H2S1).
Isotype	IgG1κ
Species Reactivity	Rat Human Mouse
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_062187
Gene Symbol	Kcnn2
Purification Method	Protein G Purified
UniProt Number	P70604
Molecular Weight	~45 kDa observed. 63.85 kDa (rat), 63.76 kDa (human isoform 1), 26.34 kDa (human isoform 2), 91.60 kDa (mouse) calculated.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in rat brain tissue lysate. Western Blotting Analysis: 2.0 µg/mL of this antibody detected SK2 (KCa2.2) in 10 µg rat brain tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 μg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABN1832	04055977329544

Documentation

Anti-SK2 (KCa2.2) Antibody, clone K78/29 MSDS

职位
物料安全数据表 (MSDS)

Anti-SK2 (KCa2.2) Antibody, clone K78/29 分析证书

标题	批号
Anti-SK2 (KCa2.2), clone K78/29 - 3239837	3239837
Anti-SK2 (KCa2.2), clone K78/29 - 3393257	3393257
Anti-SK2 (KCa2.2), clone K78/29 - 4012135	4012135
Anti-SK2 (KCa2.2), clone K78/29 -Q2650573	Q2650573

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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