Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
10-November-2010 RFH
Synonyms
Anti-Single Immunoglobulin and Toll-Interleukin 1 Receptor Domain
Application
Immunoblotting (1-5 µg/ml)
Description
Mouse monoclonal antibody supplied as ascites. Recognizes the ~32 kDa SIGIRR protein.
Background
The SIGIRR protein acts as a negative regulator of the Toll-like and IL-1R receptor signaling pathways. It attenuates the recruitment of receptor-proximal signaling components to the TLR4 receptor, probably through an TIR-TIR domain interaction with TLR4. Its extracellular domain is known to interfere with the heterodimerization of Il1R1 and IL1RAP.
Host
Mouse
Immunogen
Full-length, recombinant, human SIGIRR (aa 1-411), expressed as a GST fusion protein
Clone
3H8-2G3
Isotype
IgM
Species
human
Positive control
HepG2 cells
Form
Liquid
Formulation
Undiluted ascites
Preservative
None
Comments
Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
