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MABE927 Sigma-AldrichAnti-SAE2 Antibody, clone 7H8.1

Anti-SAE2 Antibody, clone 7H8.1 | MABE927 is an antibody against SAE2 Antibody for use in Western Blotting, Immunohistochemistry.

Anti-SAE2 Antibody, clone 7H8.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考

Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: UBA2, SAE2, UBLE1B UniProt Number: Q9UBT2

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概述

Replacement Information

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	WB, IHC	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABE927
Description	Anti-SAE2 Antibody, clone 7H8.1
Alternate Names	SUMO-activating enzyme subunit 2 Anthracycline-associated resistance ARX Ubiquitin-like 1-activating enzyme E1B Ubiquitin-like modifier-activating enzyme 2
Background Information	SUMO-activating enzyme subunit 2, also known as Anthracycline-associated resistance ARX, or Ubiquitin-like 1-activating enzyme E1B, and encoded by the gene name UBA2 or SAE2 or UBLE1B, belongs to the ubiquitin-activating E1 family. SUMOs are a family of small, related proteins (SUMO-1/2/3/4) that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation, a major regulator of protein function in cellular processes such as nuclear transport, transcriptional regulation, apoptosis and protein stability. This sumoylation is effected by the heterodimeric enzyme SAE1/SAE2 and the SUMO-1-conjugating enzyme Ubch9. The sumoylation pathway mediated by SAE1/SAE2 is distinct from other ubiquitin-like protein (Ubl) pathways.

References

Product Information
Format	Purified
Presentation	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-SAE2 Antibody, clone 7H8.1 \| MABE927 is an antibody against SAE2 Antibody for use in Western Blotting, Immunohistochemistry.
Key Applications	Western Blotting Immunohistochemistry
Application Notes	Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected SAE2 in human kidney and rat testis tissue.

Biological Information
Immunogen	GST-tagged recombinant protein corresponding to human SAE2.
Clone	7H8.1
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Isotype	IgG1κ
Species Reactivity	Human Mouse Rat
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_005490
Gene Symbol	UBA2 SAE2 UBLE1B
Purification Method	Protein G Purified
UniProt Number	Q9UBT2
Molecular Weight	~80 kDa observed. The calculated molecular weight is 72 kDa, however SAE2 has been shown as a ~70-90 kDa band in western blots (Truong, K., et al. (2012). J. Biol. Chem. 287(51):42611-42619).

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in Neuro-2a cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected SAE2 in 10 µg of Neuro-2a cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABE927	04055977126983

Documentation

Anti-SAE2 Antibody, clone 7H8.1 MSDS

职位
物料安全数据表 (MSDS)

Anti-SAE2 Antibody, clone 7H8.1 分析证书

标题	批号
Anti-SAE2, clone 7H8.1 -Q2504975	Q2504975

参考

参考概述	公共医疗ID
The Journal of biological chemistry Truong, K; Lee, TD; Li, B; Chen, Y The Journal of biological chemistry 287 42611-9 2012 显示摘要 SUMOylation occurs predominantly in the nucleus, but non-nuclear proteins can also be SUMOylated. It is unclear how intracellular trafficking of the SUMOylation enzymes is regulated to catalyze SUMOylation in different cellular compartments. Here we report that the SAE2 subunit of human SUMO activation enzyme (SAE) underwent rapid nucleocytoplasmic shuttling and its nuclear accumulation depended on SUMO modification at the C terminus. The SUMOylation sites included three Lys residues on the bipartite nuclear localization sequence (NLS) and two Lys residues outside of but adjacent to the NLS, and their SUMOylation was catalyzed by Ubc9. Because SAE2 forms a tight heterodimer with SAE1 and it controls the trafficking of the heterodimer, this study has identified the mechanism used to localize SAE to the nucleus. Similar mechanisms are likely to exist for other proteins that depend on SUMOylation for nuclear localization.	23095757

参考概述

公共医疗ID

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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