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Recognizes the ~125-130 kDa RECK protein in HeLa cells.
Catalogue Number
ST1627
Brand Family
Calbiochem®
Synonyms
Anti-Reversion-Inducing-Cysteine-Rich Protein With Kazal Motifs
Application Data
Detection of human RECK by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-RECK Rabbit pAb (Cat. No. ST1627) (1-5 µg/ml). Detection: chemiluminescence.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
09-November-2010 RFH
Synonyms
Anti-Reversion-Inducing-Cysteine-Rich Protein With Kazal Motifs
Application
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human RECK by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-RECK Rabbit pAb (Cat. No. ST1627) (1-5 µg/ml). Detection: chemiluminescence.
Description
Purified rabbit polyclonal antibody. Recognizes the ~125-130 kDa RECK protein.
Background
The protein encoded by this gene is a cysteine-rich, extracellular protein with protease inhibitor-like domains whose expression is suppressed strongly in many tumors and cells transformed by various kinds of oncogenes. In normal cells, this membrane-anchored glycoprotein may serve as a negative regulator for matrix metalloproteinase-9, a key enzyme involved in tumor invasion and metastasis.
Host
Rabbit
Immunogen species
Human
Immunogen
Full-length, human RECK (aa 1-971)
Isotype
IgG
Species
human
Positive control
HeLa cells
Negative control
293T cells
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
