数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

539229 Sigma-AldrichAnti-Protein Disulfide Isomerase Rabbit pAb

This Anti-Protein Disulfide Isomerase Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry, IP, Paraffin Sections for the detection of Protein Disulfide Isomerase.

Anti-Protein Disulfide Isomerase Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

同义词: Anti-PDI

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
B, Ca, Gp, Ht, H, Mk, M, Porcine, R, Sh, Xn	Rb	Polyclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the ~55 kDa (apparent MW) protein disulfide isomerase protein.
Catalogue Number	539229
Brand Family	Calbiochem®
Synonyms	Anti-PDI
Application Data	Detection of protein disulfide isomerase immunoblotting. Samples: Purified bovine PDI (lane 1) and whole cell lysate from HeLa cells (lane 2). Primary antibody: Anti-Protein Disulfide Isomerase Rabbit pAb (Cat. No. 539229) (1:1000). Detection: chemiluminescence.

References
References	Cribb, A.E., et al. 1997. J. Pharmacol. Exp. Ther. 282, 1064. Freedman, R.B., and Hillson, D.A. 1980. In Enzymology of post-translational modification of proteins. (Freedman R.B. and Hawkin H.C. eds.) Academic, London p. 157.

Product Information
Form	Liquid
Formulation	In PBS, 50% glycerol.
Positive control	HeLa cells, rat testis, mouse spleen
Preservative	≥0.9% sodium azide
Quality Level	MQ100

Applications
Key Applications	Immunoblotting (Western Blotting) Immunocytochemistry Immunoprecipitation Paraffin Sections
Application Notes	Immunoblotting (1:1000, colorimetric) Immunocytochemistry (see comments) Immunoprecipitation (see comments) Paraffin Sections (see comments)
Application Comments	This antibody will also work in immunocytochemistry, immunoprecipitation, and paraffin sections; the concentration must be determined for each sample analyzed. Variables associated with assay conditions will dictate the optimal working dilution.

Biological Information
Immunogen	bovine liver Protein Disulfide Isomerase
Immunogen	Bovine
Host	Rabbit
Isotype	IgG
Species Reactivity	Bovine Canine Guinea Pig Hamster Human Monkey Mouse Porcine Rat Sheep Xenopus
Antibody Type	Polyclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
539229-50ULCN	04055977268973

Documentation

Anti-Protein Disulfide Isomerase Rabbit pAb MSDS

职位
物料安全数据表 (MSDS)

Anti-Protein Disulfide Isomerase Rabbit pAb 分析证书

标题	批号
539229	输入批号

参考

参考信息概述
Cribb, A.E., et al. 1997. J. Pharmacol. Exp. Ther. 282, 1064. Freedman, R.B., and Hillson, D.A. 1980. In Enzymology of post-translational modification of proteins. (Freedman R.B. and Hawkin H.C. eds.) Academic, London p. 157.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	20-March-2012 JSW
Synonyms	Anti-PDI
Application	Immunoblotting (1:1000, colorimetric) Immunocytochemistry (see comments) Immunoprecipitation (see comments) Paraffin Sections (see comments)
Application Data	Detection of protein disulfide isomerase immunoblotting. Samples: Purified bovine PDI (lane 1) and whole cell lysate from HeLa cells (lane 2). Primary antibody: Anti-Protein Disulfide Isomerase Rabbit pAb (Cat. No. 539229) (1:1000). Detection: chemiluminescence.
Description	Protein A purified rabbit polyclonal antibody. Recognizes the ~55 kDa (apparent MW) protein disulfide isomerase protein.
Background	PDI is an abundant component of the ER that catalyzes disulfide bond formation in the biosynthesis of various secretory and cell surface proteins. It is a multi-functional polypeptide identical to the β-subunit of prolyl 4-hydroxylase and a cellular thyroid hormone-binding protein. It has two thioredoxin-like regions, each con-taining a Cys-Gly-His-Cys sequence that may represent two independently acting catalytic sites of PDI activity. The C-terminus of PDI has a KDEL sequence, which acts as retention signal for proteins of the ER lumen. PDI exists as a homodimer of ~107 kDa. Prolyl-4-hydroxylase, an α2β2 tetramer, catalyzes the formation of 4-hydroxyproline in collagen by the hydroxylation of proline residues in peptide linkages. The PDI/β-subunit of prolyl-4-hydroxylase is required for its retention in the ER.
Host	Rabbit
Immunogen species	Bovine
Immunogen	bovine liver Protein Disulfide Isomerase
Isotype	IgG
Species	Xenopus, bovine, canine, guinea pig, hamster, human, monkey, mouse, porcine, rat, sheep
Positive control	HeLa cells, rat testis, mouse spleen
Form	Liquid
Formulation	In PBS, 50% glycerol.
Preservative	≥0.9% sodium azide
Comments	This antibody will also work in immunocytochemistry, immunoprecipitation, and paraffin sections; the concentration must be determined for each sample analyzed. Variables associated with assay conditions will dictate the optimal working dilution.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Standard Handling
References	Cribb, A.E., et al. 1997. J. Pharmacol. Exp. Ther. 282, 1064. Freedman, R.B., and Hillson, D.A. 1980. In Enzymology of post-translational modification of proteins. (Freedman R.B. and Hawkin H.C. eds.) Academic, London p. 157.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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