数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

MABS1235 Sigma-AldrichAnti-Progesterone Receptor A/B Antibody, clone 488/H3

This Anti-Progesterone Receptor A/B Antibody, clone 488/H3 is validated for use in Western Blotting and Immunocytochemistry for the detection of Progesterone Receptor A/B.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
小册子
Posters

Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: PGR, NR3C3 UniProt Number: P06401

View Products on Sigmaaldrich.com

Recommended Products

概述

Replacement Information

特卖

100% Performance Guaranteed

重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, ICC	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABS1235
Description	Anti-Progesterone Receptor A/B Antibody, clone 488/H3
Alternate Names	Progesterone receptor PR Nuclear receptor subfamily 3 group C member 3 Progesterone Receptor A/B
Background Information	Progesterone receptor (UniProt P06401; also known as nuclear receptor subfamily 3 group C member 3, PR) is encoded by the PGR (also known as NR3C3) gene (Gene ID 5241) in human. The progesterone receptor (PR) is a hormone activated transcription factor, where hormone binding triggers the dissociation of heat shock and chaperone proteins from PR, leading to PR dimerization and activation. The receptor dimer then binds progestin response elements in the regulatory regions of its target genes and a multi-component complex is assembled to enable transcription. In addition, a number of kinases are reported to regulate PR activity via phosphorylation. PR can also regulate transcription without direct DNA binding by tethering to other transcription factors, such as specificity protein 1 (Sp1) or activator protein 1 (AP-1), and modulating their transcriptional activity on target genes. PR is expressed as five isoforms, including PR-B and PR-A that are produced as a result of alternate estrogen inducible promoters within the same PGR gene. PR-A lacks the first 164 amino acids of PR-B, but the two isoforms have otherwise identical sequence. PR-B and PR-A can regulate distinct subsets of target genes and studies in knockout mice suggest that PR-B predominantly regulates mammary gland development and PR-A is critical for normal uterine function.

References

Product Information
Format	Purified
Presentation	Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	This Anti-Progesterone Receptor A/B Antibody, clone 488/H3 is validated for use in Western Blotting and Immunocytochemistry for the detection of Progesterone Receptor A/B.
Key Applications	Western Blotting Immunocytochemistry
Application Notes	Immunocytochemistry Analysis: A representative lot detected exogenously expressed progesterone receptor isoform A and isoform B (PR-A & PR-B) in transfected MCF10A cells by fluorescent immunocytochemistry (Courtesy of Dr. Dean Edwards, Baylor College of Medicine, USA). Western Blotting Analysis: A representative lot detected endogenous progesterone receptor isoforms A & B (PR-A & PR-B) in human breast cancer T47D cell lysate, as well as exogenously expressed PR-A & PR-B in transfected DCIS.COM human ductal carcinoma in situ (DCIS) breast cancer cells, but not in untransfected DCIS.COM cells (Courtesy of Dr. Dean Edwards, Baylor College of Medicine, USA).

Biological Information
Immunogen	Recombinant protein corresponding to the N-terminal half fragment of human Progesterone Receptor B.
Epitope	Internal
Clone	488/H3
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Specificity	Clone 488/H3 reacts with both isoform 1 (isoform B; PR-B) and isoform 2 (isoform A; PR-A). This clone is also expected to react with isoform 5 (delta4), but not isoform 3 or 4 (PR-M).
Isotype	IgG1κ
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001189403 NP_000917
Gene Symbol	PGR NR3C3
Purification Method	Protein G Purified
UniProt Number	P06401
Molecular Weight	~110/80 kDa observed

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in human breast cancer T47D cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected both isoforms A and B (PR-A & PR-B) of progesterone receptor in human breast cancer T47D cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 μg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABS1235	04055977288407

Documentation

Anti-Progesterone Receptor A/B Antibody, clone 488/H3 MSDS

职位
物料安全数据表 (MSDS)

Anti-Progesterone Receptor A/B Antibody, clone 488/H3 分析证书

标题	批号
Anti-Progesterone Receptor A/B, -Q2607110	Q2607110
Anti-Progesterone Receptor A/B, clone 488/H3 - 3389934	3389934
Anti-Progesterone Receptor A/B, clone 488/H3 - 4030994	4030994

小册子

标题
New Products Flyer- Vol 4 Signaling Feature- NEW! Phosphohistidine Antibody

海报

标题
Post-Translational Modifications

Related Products

产品目录编号

描述

MABS1234

Anti-Progesterone Receptor B Antibody, clone 250/H11

显示价格及库存情况

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

此产品已添加到您的购物车。