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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

454578 Sigma-AldrichAnti-Pan-NMDA NR1 Mouse mAb (R1JHL)

This Anti-Pan-NMDA NR1 Mouse mAb (R1JHL) is validated for use in Immunoblotting for the detection of Pan-NMDA NR1.

Anti-Pan-NMDA NR1 Mouse mAb (R1JHL) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
小册子
Data Sheet

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
R	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the ~115 kDa NMDA receptor NR1 subunit in rat cortex tissue extracts.
Catalogue Number	454578
Brand Family	Calbiochem®

References
References	Laurie, D.J., and Seeburg, P.H. 1994. J. Neurosci. 14, 3180 Ishii, T., et al. 1993. J. Biol. Chem. 268, 2836. Monyer, H., et al. 1992. Science 256, 1217.

Product Information
Form	Lyophilized
Formulation	Lyophilized from cell culture supernatant.
Positive control	Rat cortex tissue extracts
Preservative	None
Quality Level	MQ100

Applications
Key Applications	Immunoblotting (Western Blotting) Immunoprecipitation
Application Notes	Immunoblotting (1:300, colorimetric) Immunoprecipitation (3 µg/200 µg lysate)
Application Comments	Does not cross-react with other NMDAR subunits. For immunoblotting, dilute in TBST containing 5% nonfat milk and 0.05% sodium azide. Antibody may be used up to 5 times in immunoblotting experiments without significant loss in signal strength. Store diluted antibody at 4°C between uses. Variables associated with assay conditions will dictate the proper working dilution.

Biological Information
Immunogen	a recombinant fusion protein consisting of amino acids 1-564 of rat NMDA NR1
Immunogen	Rat
Clone	R1JHL
Host	Mouse
Isotype	IgG
Species Reactivity	Rat
Antibody Type	Monoclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Ok to freeze
Special Instructions	Reconstitute in 50 µl PBS (137 mM NaCl, 7.5 mM Na₂HPO₄, 2.7 mM KCl, 1.5 mM KH₂P0₄, pH 7.4)Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
454578-15UGCN	04055977204155

Documentation

Anti-Pan-NMDA NR1 Mouse mAb (R1JHL) MSDS

职位
物料安全数据表 (MSDS)

Anti-Pan-NMDA NR1 Mouse mAb (R1JHL) 分析证书

标题	批号
454578	输入批号

参考

参考信息概述
Laurie, D.J., and Seeburg, P.H. 1994. J. Neurosci. 14, 3180 Ishii, T., et al. 1993. J. Biol. Chem. 268, 2836. Monyer, H., et al. 1992. Science 256, 1217.

小册子

标题
Pathways and Biomarkers of Glutamatergic Synapse Flyer

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	05-August-2008 JSW
Application	Immunoblotting (1:300, colorimetric) Immunoprecipitation (3 µg/200 µg lysate)
Description	Mouse monoclonal antibody lyophilized from cell culture supernatant. Recognizes the ~115 kDa subunit of the NMDAR (N-methyl-D-aspartate receptor) protein.
Background	The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. NR1 can form NMDA-activated channels when expressed in oocytes, but the currents are larger when combined with one or more of the NMDAR2 (NR2 A-D) subunits.
Host	Mouse
Immunogen species	Rat
Immunogen	a recombinant fusion protein consisting of amino acids 1-564 of rat NMDA NR1
Clone	R1JHL
Isotype	IgG
Species	rat
Positive control	Rat cortex tissue extracts
Form	Lyophilized
Formulation	Lyophilized from cell culture supernatant.
Preservative	None
Comments	Does not cross-react with other NMDAR subunits. For immunoblotting, dilute in TBST containing 5% nonfat milk and 0.05% sodium azide. Antibody may be used up to 5 times in immunoblotting experiments without significant loss in signal strength. Store diluted antibody at 4°C between uses. Variables associated with assay conditions will dictate the proper working dilution.
Storage	+2°C to +8°C
Do Not Freeze	Ok to freeze
Special Instructions	Reconstitute in 50 µl PBS (137 mM NaCl, 7.5 mM Na₂HPO₄, 2.7 mM KCl, 1.5 mM KH₂P0₄, pH 7.4)Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Toxicity	Standard Handling
References	Laurie, D.J., and Seeburg, P.H. 1994. J. Neurosci. 14, 3180 Ishii, T., et al. 1993. J. Biol. Chem. 268, 2836. Monyer, H., et al. 1992. Science 256, 1217.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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