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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

NA03 Sigma-AldrichAnti-PCNA (Ab-1) Mouse mAb (PC10)

This Anti-PCNA (Ab-1) Mouse mAb (PC10) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation Paraffin Sections for the detection of PCNA (Ab-1).

Anti-PCNA (Ab-1) Mouse mAb (PC10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet
Citations

同义词: Anti-Proliferating Cell Nuclear Antigen

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
H, M, R, Yeast, Zebrafish	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the ~37 kDa PCNA protein in HeLa cells as well as in tonsil or normal colon tissue.
Catalogue Number	NA03
Brand Family	Calbiochem®
Synonyms	Anti-Proliferating Cell Nuclear Antigen

References
References	Waseem, N.H. and Lane, D.P. 1990. J. Cell. Sci. 96, 121. Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA 86 3189. Bravo, R., et al. 1987. Nature (London) 326, 515. Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol. 105, 1549. Miyachi, K., et al. 1987. J. Immunol. 121, 2228.

Product Information
Form	Liquid
Formulation	In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Positive control	HeLa cells, HL-60 cells, tonsil tissue, or normal colon tissue
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Key Applications	Immunoblotting (Western Blotting) Immunofluorescence Not Immunoprecipitation Paraffin Sections
Application Notes	Immunoblotting (2.5 µg/ml) Immunofluorescence (1-5 µg/ml) Immunoprecipitation (not recommended with unconjugated antibody, see comments) Paraffin Sections (2.5 µg/ml, no pre-treatment required, see comments)
Application Comments	Staining shows an intense nuclear pattern. Not recommended for immunoprecipitation because in some samples a ~37 kDa protein will be precipitated that is not PCNA. Generally, no pre-treatment is needed to stain paraffin sections, but staining may be enhanced in some samples by pretreating with 4 N HCl for 10 min at 37°C followed by 3 washes in water. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	recombinant PCNA
Epitope	Within amino acids 112-121
Clone	PC10
Host	Mouse
Isotype	IgG_2a
Species Reactivity	Human Mouse Rat Yeast Zebrafish
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Yes

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
NA03-200UGCN	04055977227642

Documentation

Anti-PCNA (Ab-1) Mouse mAb (PC10) MSDS

职位
物料安全数据表 (MSDS)

Anti-PCNA (Ab-1) Mouse mAb (PC10) 分析证书

标题	批号
NA03	输入批号

参考

参考信息概述
Waseem, N.H. and Lane, D.P. 1990. J. Cell. Sci. 96, 121. Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA 86 3189. Bravo, R., et al. 1987. Nature (London) 326, 515. Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol. 105, 1549. Miyachi, K., et al. 1987. J. Immunol. 121, 2228.

引用

标题

Goessling, et al. 2008. Developmental Biology 320, 161.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	04-September-2007 RFH
Synonyms	Anti-Proliferating Cell Nuclear Antigen
Application	Immunoblotting (2.5 µg/ml) Immunofluorescence (1-5 µg/ml) Immunoprecipitation (not recommended with unconjugated antibody, see comments) Paraffin Sections (2.5 µg/ml, no pre-treatment required, see comments)
Description	Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0-Ag14 mouse myeloma cells. Recognizes the ~37 kDa PCNA protein.
Background	The proliferating cell nuclear antigen (PCNA) is a 37 kDa molecular weight protein also known as cyclin. The protein has also been identified as the polymerase δ accessory protein and is detected in a cell cycle dependent manner. In early S phase, PCNA has a very granular distribution and is absent from the nucleoli. At late S phase, PCNA is prominent in the nucleoli. In cells fixed with organic solvents, the PCNA is seen to be strongly associated in the nuclear regions where DNA synthesis is occurring, whereas in cells fixed with aldehydes, the staining is more diffuse but intense and occurs throughout the cell cycle. This is due to the presence of two basic forms of the PCNA protein: a soluble form which is sensitive to organic fixation and not involved in replication and a second form which is insoluble and associated with on-going DNA synthesis. PCNA is a very conserved protein present not only in mammals but also in plant cells. Patients suffering from SLE have been shown to have auto antibodies against PCNA.
Host	Mouse
Immunogen	recombinant PCNA
Epitope	Within amino acids 112-121
Clone	PC10
Isotype	IgG_2a
Species	human, mouse, rat, yeast, zebrafish
Positive control	HeLa cells, HL-60 cells, tonsil tissue, or normal colon tissue
Form	Liquid
Formulation	In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	Staining shows an intense nuclear pattern. Not recommended for immunoprecipitation because in some samples a ~37 kDa protein will be precipitated that is not PCNA. Generally, no pre-treatment is needed to stain paraffin sections, but staining may be enhanced in some samples by pretreating with 4 N HCl for 10 min at 37°C followed by 3 washes in water. Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Yes
Toxicity	Standard Handling
References	Waseem, N.H. and Lane, D.P. 1990. J. Cell. Sci. 96, 121. Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA 86 3189. Bravo, R., et al. 1987. Nature (London) 326, 515. Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol. 105, 1549. Miyachi, K., et al. 1987. J. Immunol. 121, 2228.
Citation	Goessling, et al. 2008. Developmental Biology 320, 161.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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