Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
05-November-2010 RFH
Synonyms
Anti-Phosphenolpyruvate Carboxykinase 1
Application
ELISA (see comments) Immunoblotting (1-5 µg/ml) Immunocytochemistry (10 µg/ml)
Application Data
Detection of human PCK1 by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-PCK1 Mouse mAb (3E4) (Cat. No. ST1508) (1-5 µg/ml). Detection: chemiluminescence. Detection of human PCK1 by immunocytochemistry. Sample: HepG2 cells fixed in paraformaldehyde. Primary antibody: Anti-PCK1 Mouse mAb (3E4) (Cat. No. ST1508) (10 µg/ml). Detection: fluorescence.
Description
Purified mouse monoclonal antibody. Recognizes the ~70 kDa PCK1 protein.
Background
PCK1 is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency.
Host
Mouse
Immunogen
Full-length, recombinant human PCK1 (aa 1-623), expressed as a GST fusion protein
Clone
3E4
Isotype
IgG₁
Species
human
Positive control
HepG2 cells
Negative control
293T cells
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
