Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
A recombinant polypeptide corresponding to amino acids of 85-195 human PAPPA, expressed as a GST fusion protein
Clone
1G3
Host
Mouse
Isotype
IgG2b
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
10-November-2010 RFH
Synonyms
Anti-Pregnancy-Associated Plasma Protein A, Anti-Pappalysin 1
Application
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human PAPPA by immunoblotting. Sample: Partial recombinant protein. Primary antibody: Anti-PAPPA Mouse mAb (1G3) (Cat. No. ST1714) (1-5 µg/ml). Detection: chemiluminescence.
Description
Purified mouse monoclonal antibody. Recognizes the PAPPA protein.
Background
This gene encodes a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). It is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses.
Host
Mouse
Immunogen
A recombinant polypeptide corresponding to amino acids of 85-195 human PAPPA, expressed as a GST fusion protein
Clone
1G3
Isotype
IgG2b
Species
human
Form
liquid
Formulation
In PBS, pH 7.2.
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
