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04-1467 Sigma-AldrichAnti-PABP 1 Antibody, clone 10E10

Anti-PABP 1, clone 10E10 is a Mouse Monoclonal Antibody for detection of PABP 1 also known as PABP 1, Poly(A)-binding protein 1, poly(A) binding protein cytoplasmic 1 & has been validated in WB, ICC & IP.

Anti-PABP 1 Antibody, clone 10E10 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: PABPC1, PAB1, PABP, PABP1, PABPC2, PABPL1 UniProt Number: P11940

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, ICC, IP	M	Purified	Monoclonal Antibody

Description
Catalogue Number	04-1467
Replaces	05-847
Description	Anti-PABP 1 Antibody, clone 10E10
Alternate Names	PABP 1 Poly(A)-binding protein 1 poly(A) binding protein, cytoplasmic 1 poly(A) binding protein, cytoplasmic 2 poly(A)-binding protein, cytoplasmic 2
Background Information	Poly(A)-binding protein (PABP 1) is an abundant protein that interacts with the 3' poly(A) tail of most eukaryotic mRNAs. In vivo this protein is involved in many aspects of mRNA metabolism, including mRNA stability, translationally coupled mRNA turnover, protein biosynthesis, and ribosome biogenesis. PABP1 shuttles between the cytoplasm and the nucleus and may bind to cytoplasmic RNA sequences other than poly(A). PABP1 accumulates in the nucleus if transcription is inhibited, suggesting that nuclear export is coupled to active transcription.

References

Product Information
Format	Purified
Control	HeLa cell lysate
Presentation	Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-PABP 1, clone 10E10 is a Mouse Monoclonal Antibody for detection of PABP 1 also known as PABP 1, Poly(A)-binding protein 1, poly(A) binding protein cytoplasmic 1 & has been validated in WB, ICC & IP.
Key Applications	Western Blotting Immunocytochemistry Immunoprecipitation
Application Notes	Immunoprecipitation: Cited in Thoma, C., et al. (2008) RNA. 14:1579-1589, and Buchet-Poyau, K., et al.(2007) Nuc. Acids Res. 35:1289-1300 using a representative lot. Immunocytochemistry: Cited in Afonina, E, et al. (1998) J. Biol. Chem., 273: 13015-21 (1998) using a representative lot.

Biological Information
Immunogen	Recombinant protein corresponding to human PABP 1.
Epitope	Unknown
Clone	10E10
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes PABP 1.
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_002559.2
Entrez Gene Summary	The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation. In humans, the PABPs comprise a small nuclear isoform and a conserved gene family that displays at least 3 functional proteins: PABP1 (PABPC1), inducible PABP (iPABP, or PABPC4; MIM 603407), and PABP3 (PABPC3; MIM 604680). In addition, there are at least 4 pseudogenes, PABPCP1 to PABPCP4.[supplied by OMIM]
Gene Symbol	PABPC1 PAB1 PABP PABP1 PABPC2 PABPL1
Purification Method	Protein G Purified
UniProt Number	P11940
UniProt Summary	FUNCTION: Binds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of mRNA metabolism such as pre- mRNA splicing. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. SIZE: 636 amino acids; 70671 Da SUBUNIT: Component of a multi subunit autoregulatory ribonucleoprotein complex (ARC), at least composed of IGF2BP1, PABPC1 and CSDE1. Interacts with IGF2BP1. Part of a complex associated with the FOS mCRD domain and consisting of HNRPD, SYNCRIP, PAIP1 and CSDE1/UNR. Interacts with the PABPC1- interacting motif-1 (PAM1) and -2 (PAM2) of PAIP1 and PAIP2. Interacts with PAIP1 with a 1:1 stoichiometry and with PAIP2 with a 1:2 stoichiometry. The interaction with CSDE1 is direct and RNA- independent. Found in a mRNP complex with YBX2. Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8 (By similarity). SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Shuttles between the cytoplasm and the nucleus. TISSUE SPECIFICITY: Ubiquitous. DOMAIN: The RNA-binding domains RRM1 and RRM2 and the C-terminus (last 138 amino acids) regions interact with the PABPC1- interacting motif-1 (PAM1) and -2 (PAM2) of PAIP1, respectively. & The RNA-binding domains RRM2 and RRM3 and the C-terminus (last 138 amino acids) regions interact with the PABPC1- interacting motif-1 (PAM1) and -2 (PAM2) of PAIP2, respectively. PTM: Methylated by CARM1. & Arg-493 is dimethylated, probably to asymmetric dimethylarginine. SIMILARITY: Contains 1 PABC domain. & Contains 4 RRM (RNA recognition motif) domains.
Molecular Weight	~ 71 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: 0.5 µg/ml of this antibody detected PABP 1 in 10 µg of HeLa cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
04-1467	04053252276545

Documentation

Anti-PABP 1 Antibody, clone 10E10 MSDS

职位
物料安全数据表 (MSDS)

Anti-PABP 1 Antibody, clone 10E10 分析证书

标题	批号
Anti-PABP 1, clone 10E10 - 2433424	2433424
Anti-PABP 1, clone 10E10 - 1977632	1977632
Anti-PABP 1, clone 10E10 - 2030042	2030042
Anti-PABP 1, clone 10E10 - 2349625	2349625
Anti-PABP 1, clone 10E10 - 3698145	3698145
Anti-PABP 1, clone 10E10 - 3984056	3984056
Anti-PABP 1, clone 10E10 - NG1817554	NG1817554
Anti-PABP 1, clone 10E10 - NRG1693920	NRG1693920
Anti-PABP 1, clone 10E10 -2703668	2703668
Anti-PABP 1, clone 10E10 -2798614	2798614

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种类

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