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Recognizes the ~130 kDa OPA1 protein in human colon tissue.
Catalogue Number
ST1587
Brand Family
Calbiochem®
Synonyms
Anti-Optic Atrophy 1
Application Data
Detection of human OPA1 by immunoblotting. Sample: Whole tissue extract from human colon. Primary antibody: Anti-OPA1 Rabbit pAb (Cat. No. ST1587) (1-5 µg/ml). Detection: chemiluminescence.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
09-November-2010 RFH
Synonyms
Anti-Optic Atrophy 1
Application
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human OPA1 by immunoblotting. Sample: Whole tissue extract from human colon. Primary antibody: Anti-OPA1 Rabbit pAb (Cat. No. ST1587) (1-5 µg/ml). Detection: chemiluminescence.
Description
Purified rabbit polyclonal antibody. Recognizes the ~130 kDa OPA1 protein.
Background
OPA1 is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. It is a component of the mitochondrial network. Mutations in this gene have been associated with optic atrophy type 1, which is a dominantly inherited optic neuropathy resulting in progressive loss of visual acuity, leading in many cases to legal blindness. Multiple transcript variants encoding different isoforms have been found for this gene.
Host
Rabbit
Immunogen species
Human
Immunogen
Full-length, human OPA1 (aa 1-960)
Isotype
IgG
Species
human
Positive control
Human colon tissue
Negative control
293T cells
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
