Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Yolanda Fernandez, et al. (2006) Chemical Blockage of the Proteasome Inhibitory Function of Bortezomib: Impact on Tumor Cell Death. Journal of Biological Chemistry281, 1107-1118.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
08-October-2007 JSW
Application
Immunoblotting (1-2 µg/ml, chemiluminescence)
Application Data
Detection of human Noxa by immunoblotting. Sample: Whole cell lysate from RL-7 cells. Primary antibody: Anti-Noxa Mouse mAb (114C307) (Cat. No. OP180) (2 µg/ml). Detection: chemiluminescence.
Description
Purified mouse monoclonal antibody. Recognizes the ~11 kDa Noxa protein.
Background
Noxa, a BH3 member of the Bcl-2 family, has recently been identified in human and mouse. Expression of the Noxa gene involves direct activation of its promoter by p53. Increased expression of Noxa occurs in normal thymocytes, but does not occur in p53-deficient thymocytes. Coimmunoprecipitation data suggests that Noxa may interact with other members of the Bcl-2 family, including Bcl-XL and Mcl-1. Data has also shown that blocking the endogenous Noxa induction results in the suppression of apoptosis. Treating cells with Noxa antisense oligonucleotides blocks radiation-induced apoptosis, and this suggests that Noxa may be a mediator of p53-dependent apoptosis.
Host
Mouse
Immunogen species
Human
Immunogen
a recombinant protein consisting of human Noxa fused to GST
Clone
114C307
Isotype
IgG₁
Species
human
Positive control
RL-7 cell lysate
Negative control
Normal mouse serum
Form
Liquid
Formulation
In PBS, 0.05% BSA.
Concentration Label
Please refer to vial label for lot-specific concentration
Preservative
≤0.1% sodium azide
Comments
Antibody should be titrated for optimal results in individual system.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C;)
Toxicity
Standard Handling
References
Oda, E., et al. 2000. Science288, 1053.
Citation
Yolanda Fernandez, et al. (2006) Chemical Blockage of the Proteasome Inhibitory Function of Bortezomib: Impact on Tumor Cell Death. Journal of Biological Chemistry281, 1107-1118.
