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MABF937 Sigma-AldrichAnti-Notch 3 Antibody, ECD, clone 2G8

Anti-Notch 3 Antibody, ECD, clone 2G8 is an antibody against Notch 3 for use in Immunohistochemistry (Paraffin), Immunofluorescence, Western Blotting.

Anti-Notch 3 Antibody, ECD, clone 2G8 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Inflammation & Immunology Research Sub-Category: Nervous System Gene Symbol: NOTCH3, CASIL, CADASIL, IMF2 UniProt Number: Q9UM47

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Replacement Information

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	IH(P), IF, WB	R	Purified	Monoclonal Antibody

Description
Catalogue Number	MABF937
Description	Anti-Notch 3 Antibody, ECD, clone 2G8
Alternate Names	Neurogenic locus notch homolog protein 3 Notch 3 Notch homolog 3 Notch 3 ECD
Background Information	Neurogenic locus notch homolog protein 3 (UniProt Q9UM47; also known as Notch 3, Notch homolog 3) is encoded by the NOTCH3 (also known as CASIL, CADASIL, IMF2) gene (Gene ID 4854) in human. Notch 3 is a transmembrane receptor required for arterial differentiation and maturation of vascular smooth muscle cells in small arteries. Notch 3 is initially produced with a signal peptide (a.a. 1-39), the removal of which yields the 2282-amino acid Notch 3 (a.a. 40-2321), which undergoes a constitutive proteolytical cleavage, generating the extracellular fragment (Notch3-ECD; a.a. 40-1628) and the transmembrane/cytosolic (Notch3-TMIC; a.a. 1629-2321) fragment that remain associated at the cell surface to form a heterodimeric receptor. The ECD fragment contains all 34 EGF-like domains and the three Lin/Notch (LNR) repeats, while the extracellular portion of TMIC contains 5 ankyrin repeats. Mutations in the NOTCH3 gene have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). Pathogenic mutations predominantly affect cysteine residues within EGF-like repeats of Notch3-ECD, leading to Notch3-ECD multimerization and accumulation in the tunica media of vessel walls.

References

Product Information
Format	Purified
Presentation	Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-Notch 3 Antibody, ECD, clone 2G8 is an antibody against Notch 3 for use in Immunohistochemistry (Paraffin), Immunofluorescence, Western Blotting.
Key Applications	Immunohistochemistry (Paraffin) Immunofluorescence Western Blotting
Application Notes	Immunohistochemistry Analysis: 20 µg/mL of this antibody from a representative lot detected Notch 3 extracellular domain (ECD) deposits in blood vessels of CADASIL patients brain tissue sections (Courtesy of Institute for Stroke and Dementia Research, Clinical center of the Ludwig-Maximilians University Munich, Germany). Immunohistochemistry Analysis: A representative lot detected the aggregated Notch 3 extracellular domain (ECD) deposits in brain blood vessels of CADASIL patients by immunohistochemistry staining of paraformaldehyde-fixed, paraffin-embedded brain tissue sections (Kast, J., et al. (2014). Acta Neuropathol. Commun. 2:96). Immunofluorescence Analysis: A representative lot detected the aggregated Notch 3 extracellular domain (ECD) deposits in brain blood vessels of CADASIL patients by fluorescent immunohistochemistry staining of paraformaldehyde-fixed frozen brain tissue sections. The Notch3-EDC immunoreactivity was seen co-localized with that of LTBP-1, but not fibrillin-1 (Kast, J., et al. (2014). Acta Neuropathol. Commun. 2:96). Western Blotting Analysis: A representative lot detected an elevated accumulation of aggregated Notch 3 extracellular domain (ECD) in brain blood vessel extracts from CADASIL patients when comparing to vessel extracts from non-diseased brains (Kast, J., et al. (2014). Acta Neuropathol. Commun. 2:96).

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to a sequence from the EGF-like domains 33-34 region of human Notch 3 ECD.
Epitope	Extracellular EGF-like domains 33-34.
Clone	2G8
Concentration	Please refer to lot specific datasheet.
Host	Rat
Specificity	Clone 2G8 reacts with unprocessed Notch 3 and the extracellular (Notch 3-ECD; a.a. 40-1628) fragment, but not the transmembrane/cytosolic fragment (Notch 3-TMIC, also called Notch 3 extracellular truncation; a.a. 1629-2321) or the Notch 3 intracellular fragment (a.a. 1662-2321).
Isotype	IgG2aκ
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_000426
Gene Symbol	NOTCH3 CASIL CADASIL IMF2
Purification Method	Protein G Purified
UniProt Number	Q9UM47
Molecular Weight	243.6 kDa (Pre-form), 239.5 kDa (mature, unprocessed), 167.13 kDa (Notch 3-ECD) calculated. ~250 kDa (Notch 3-ECD) reported (Kast, J., et al. (2014). Acta Neuropathol. Commun. 2:96).

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Identity Confirmation by Isotyping Test. Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be IgG2aκ.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 μg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABF937	04055977330311

Documentation

Anti-Notch 3 Antibody, ECD, clone 2G8 MSDS

职位
物料安全数据表 (MSDS)

Anti-Notch 3 Antibody, ECD, clone 2G8 分析证书

标题	批号
Anti-Notch 3, ECD, clone 2G8 - 3234483	3234483
Anti-Notch 3, ECD, clone 2G8 - 3886044	3886044
Anti-Notch 3, ECD, clone 2G8 - 3933242	3933242
Anti-Notch 3, ECD, clone 2G8 - 4094282	4094282
Anti-Notch 3, ECD, clone 2G8 -Q2659003	Q2659003
Anti-Notch 3, ECD, clone 2G8 Monoclonal Antibody	3117566
Anti-Notch 3, ECD, clone 2G8 Monoclonal Antibody	3316666

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种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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