Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Recognizes the ~40-45 kDa NDE1 protein in HeLa cells.
Catalogue Number
ST1699
Brand Family
Calbiochem®
Synonyms
Anti-nudE, Anti-Nuclear Distribution Gene E Homolog1
Application Data
Detection of human NDE1 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-NDE1 Mouse mAb (2G11-1C11) (Cat. No. ST1699) (1-5 µg/ml). Detection: chemiluminescence. Detection of human NDE1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-NDE1 Mouse mAb (2G11-1C11) (Cat. No. ST1699) (10 µg/ml). Detection: fluorescence.
References
References
Bradshaw, N.J., et al. 2009. Neurosci. Lett.449, 228.
ELISA (see coments) Immunoblotting (1-4 µg/ml) Immunocytochemistry (10 µg/ml)
Application Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
Full-length, recombinant, human NDE1 (aa 1-336), expressed as a GST fusion protein
Clone
2G11-1C11
Host
Mouse
Isotype
IgG₁
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Bradshaw, N.J., et al. 2009. Neurosci. Lett.449, 228.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
10-November-2010 RFH
Synonyms
Anti-nudE, Anti-Nuclear Distribution Gene E Homolog1
Application
ELISA (see coments) Immunoblotting (1-4 µg/ml) Immunocytochemistry (10 µg/ml)
Application Data
Detection of human NDE1 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-NDE1 Mouse mAb (2G11-1C11) (Cat. No. ST1699) (1-5 µg/ml). Detection: chemiluminescence. Detection of human NDE1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-NDE1 Mouse mAb (2G11-1C11) (Cat. No. ST1699) (10 µg/ml). Detection: fluorescence.
Description
Purified mouse monoclonal antibody. Recognizes the ~40-45 kDa NDE1 protein.
Background
This gene encodes a member of the nuclear distribution E (NudE) family of proteins. The encoded protein is localized at the centrosome and interacts with other centrosome components as part of a multiprotein complex that regulates dynein function. This protein plays an essential role in microtubule organization, mitosis and neuronal migration. Alternative splicing results in multiple transcript variants.
Host
Mouse
Immunogen
Full-length, recombinant, human NDE1 (aa 1-336), expressed as a GST fusion protein
Clone
2G11-1C11
Isotype
IgG₁
Species
human
Positive control
HeLa cells
Form
liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Toxicity
Regulatory Review
References
Bradshaw, N.J., et al. 2009. Neurosci. Lett.449, 228.
