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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

NE1019 Sigma-AldrichAnti-Myelin Basic Protein Mouse mAb (SMI-99)

This Anti-Myelin Basic Protein Mouse mAb (SMI-99) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Frozen Sections, Paraffin Sections for the detection of Myelin Basic Protein.

Anti-Myelin Basic Protein Mouse mAb (SMI-99) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
Gp, Ma	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the ~21 kDa myelin basic protein in rat brain tissue. Does not recognize the 14 kDa form. Recognizes most mammalian species.
Catalogue Number	NE1019
Brand Family	Calbiochem®
Application Data	Detection of rat myelin basic protein by staining frozen sections. Sample: Rat brain. Primary antibody: Anti-Myelin Basic Protein Mouse mAb (SMI-99) (Cat. No. NE1019) (1:1000). Detection: fluorescence (green) with Hoechst 33342 counterstain.

References
References	Evers, P. and Uylings, H.B. 1997. J. Neurosci. Methods 72, 197. Shin, R.W., et al. 1991. Lab. Invest. 64, 693.

Product Information
Form	Liquid
Formulation	In PBS.
Positive control	Rat brain
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Key Applications	Enzyme-Linked Immunosorbent Assay Frozen Sections Immunoblotting (Western Blotting) Immunocytochemistry Paraffin Sections
Application Notes	ELISA (1:1000) Immunoblotting (1:1000) Immunocytochemistry (1:1000) Frozen Sections (1:1000) Paraffin Sections (1:1000)
Application Comments	Recognizes MBP from most mammalian species. Does not recognize the ~14 kDa form of MBP. Detects developing and adult myelin and developing oligodendrocytes. Also distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain tissue sections. In combination with Anti-Myelin Basic Protein Mouse mAb (SMI-91) (Cat. No. NE1020) and/or Anti-Myelin Basic Protein Mouse mAb (SMI-94) (Cat. No. NE1018) this antibody is useful for immunocytochemical studies on the progression of normal and pathologic myelination. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	human myelin basic protein
Immunogen	Human
Epitope	Within amino acids 131-136
Clone	SMI-99
Host	Mouse
Isotype	IgG_2b
Species Reactivity	Guinea Pig Mammals
Antibody Type	Monoclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
NE1019-100ULCN	04055977209884

Documentation

Anti-Myelin Basic Protein Mouse mAb (SMI-99) MSDS

职位
物料安全数据表 (MSDS)

Anti-Myelin Basic Protein Mouse mAb (SMI-99) 分析证书

标题	批号
NE1019	输入批号

参考

参考信息概述
Evers, P. and Uylings, H.B. 1997. J. Neurosci. Methods 72, 197. Shin, R.W., et al. 1991. Lab. Invest. 64, 693.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	02-July-2008 JSW
Application	ELISA (1:1000) Immunoblotting (1:1000) Immunocytochemistry (1:1000) Frozen Sections (1:1000) Paraffin Sections (1:1000)
Application Data	Detection of rat myelin basic protein by staining frozen sections. Sample: Rat brain. Primary antibody: Anti-Myelin Basic Protein Mouse mAb (SMI-99) (Cat. No. NE1019) (1:1000). Detection: fluorescence (green) with Hoechst 33342 counterstain.
Description	Purified mouse monoclonal antibody. Recognizes the ~21 kDa myelin basic protein (MBP).
Background	The myelin sheath is essential for rapid and effective propagation of action potential within the axon. Mutations identified in several myelin-related genes reportedly lead to severe demyelinating neuropathies. Recent evidence also suggests that the autoimmune disease Multiple Sclerosis (MS) is mediated by T cells with specificity for antigens in the myelin sheath. Although myelin in the central nervous system (CNS) and peripheral nervous system (PNS) is structurally similar, it is chemically distinct since oligodendrocytes form myelin in the CNS whereas Schwann cells generate PNS myelin. Myelin proteolipid protein (PLP) is the predominant integral membrane protein in mammalian CNS myelin. In contrast, myelin basic protein (MBP) is localized to the myelin cytoplasm and is highly conserved among species. Both MBP and PLP mediate myelin compaction, whereas thickness is regulated by MBP. Several other myelin proteins have been identified to play distinct or partially overlapping functions in the formation and maintenance of the myelin sheath, including P0, myelin-associated glycoprotein (MAG), peripheral myelin protein (PMP22), and Connexin 32 (Cx32). The development of the myelin sheath has been a good model for understanding the mechanisms of neuron-glia interaction.
Host	Mouse
Immunogen species	Human
Immunogen	human myelin basic protein
Epitope	Within amino acids 131-136
Clone	SMI-99
Isotype	IgG_2b
Species	not chicken, guinea pig (weakly), mammalian, not porcine
Positive control	Rat brain
Form	Liquid
Formulation	In PBS.
Preservative	≤0.1% sodium azide
Comments	Recognizes MBP from most mammalian species. Does not recognize the ~14 kDa form of MBP. Detects developing and adult myelin and developing oligodendrocytes. Also distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain tissue sections. In combination with Anti-Myelin Basic Protein Mouse mAb (SMI-91) (Cat. No. NE1020) and/or Anti-Myelin Basic Protein Mouse mAb (SMI-94) (Cat. No. NE1018) this antibody is useful for immunocytochemical studies on the progression of normal and pathologic myelination. Antibody should be titrated for optimal results in individual systems.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Standard Handling
References	Evers, P. and Uylings, H.B. 1997. J. Neurosci. Methods 72, 197. Shin, R.W., et al. 1991. Lab. Invest. 64, 693.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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