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07-013-25UG Sigma-AldrichAnti-MeCP2

Use Anti-MeCP2 Antibody (rabbit polyclonal antibody) validated in WB to detect MeCP2 also known as MeCP-2 protein, mental retardation X-linked 16, mental retardation X-linked 79, methyl CpG binding protein 2.

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Description
Catalogue Number	07-013-25UG
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-MeCP2
Alternate Names	MeCP-2 protein mental retardation, X-linked 16 mental retardation, X-linked 79 methyl CpG binding protein 2 methyl CpG binding protein 2 (Rett syndrome)
Background Information	NA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, imprinting, and X chromosome inactivation. Methyl CpG binding proteins (MeCPs) specifically recognize methylated regions of DNA and repress transcription both directly and by association with known corepressor proteins which include members of the histone deacetylase protein families.

References

Product Information
Format	Purified
Control	Mouse Brain lysate.
Presentation	Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Use Anti-MeCP2 Antibody (rabbit polyclonal antibody) validated in WB to detect MeCP2 also known as MeCP-2 protein, mental retardation X-linked 16, mental retardation X-linked 79, methyl CpG binding protein 2.
Key Applications	Western Blotting

Biological Information
Immunogen	KLH-conjugated, synthetic peptide corresponding to amino acids 465-478 of mouse MeCP2 (C-PRPNREEPVDSRTP) with an N-terminal cysteine added for conjugation purposes.
Concentration	Please refer to lot specific datasheet.
Host	Rabbit
Specificity	Recognizes MeCP2, Mr 75 kDa. A protein at ~75 kDa was also observed in HeLa nuclear extract. This protein was competed by pre-incubation of the antibody with the immunogenic peptide, suggesting that some HeLa strains contain MeCP2 or a related protein, contrary to a previous report.
Isotype	IgG
Species Reactivity	Mouse Rat
Species Reactivity Note	Mouse and rat. Predicted to cross-react with human based on the conservation of the immunogenic sequence.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_004983
Entrez Gene Summary	DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.
Gene Symbol	AUTSX3 DKFZp686A24160 MRX16 MRX79 MRXS13 MRXSL MeCP2 OTTHUMP00000026021 PPMX RTS RTT
Purification Method	Protein A Purfied
UniProt Number	P51608
UniProt Summary	FUNCTION: SwissProt: P51608 # Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. SIZE: 486 amino acids; 52441 Da SUBUNIT: Interacts with FNBP3 (By similarity). SUBCELLULAR LOCATION: Nucleus. Note=Colocalized with methyl-CpG in the genome. TISSUE SPECIFICITY: Present in all adult somatic tissues tested. PTM: Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation (By similarity). DISEASE: SwissProt: P51608 # Defects in MECP2 may be a cause of Angelman syndrome (AS) [MIM:105830]; also known as happy puppet syndrome. AS is a neurodevelopmental disorder characterized by severe mental retardation, absent speech, ataxia, sociable affect and dysmorphic facial features. AS and Rett syndrome have overlapping clinical features. & Defects in MECP2 are the cause of mental retardation syndromic X-linked type 13 (MRXS13) [MIM:300055]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRXS13 patients manifest mental retardation associated with other variable features such as spasticity, episodes of manic depressive psychosis, increased tone and macroorchidism. & Defects in MECP2 are the cause of Rett syndrome (RTT) [MIM:312750]. RTT is an X-linked dominant disease, it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Patients appear to develop normally until 6 to 18 months of age, then gradually lose speech and purposeful hand movements and develop microcephaly, seizures, autism, ataxia, intermittent hyperventilation, and stereotypic hand movements. After initial regression, the condition stabilizes and patients usually survive into adulthood. & Defects in MECP2 may be the cause of susceptibility to X- linked autism 3 (AUTSX3) [MIM:300496]. AUTSX3 is a pervasive developmental disorder (PDD), prototypically characterized by impairments in reciprocal social interaction and communication, restricted and stereotyped patterns of interests and activities, and the presence of developmental abnormalities by 3 years of age. & Defects in MECP2 may be a cause of nonprogressive encephalopathy with neonatal onset [MIM:300005]. & A chromosomal duplication involving MECP2 is the cause of mental retardation syndromic X-linked Lubs type (MRXSL) [MIM:300260]. Increased dosage of MECP2 appears to be responsible for the mental retardation phenotype. The main features present in affected males are severe to profound mental retardation with onset at birth, axial and facial hypotonia, progressive spasticity predominantly at the lower limbs, seizures and recurrent infections. SIMILARITY: SwissProt: P51608 ## Contains 2 A.T hook DNA-binding domains. & Contains 1 MBD (methyl-CpG-binding) domain.
Molecular Weight	~75 kDa observed

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blot on Mouse Brain lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	25 μg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
07-013-25UG	04054839342479

Documentation

Anti-MeCP2 MSDS

职位
物料安全数据表 (MSDS)

Anti-MeCP2 分析证书

标题	批号
Anti-MeCP2 - 3318568	3318568
Anti-MeCP2 - 3433930	3433930
Anti-MeCP2 - 3821641	3821641
Anti-MeCP2 - 3940280	3940280
Anti-MeCP2 - 4074920	4074920
Anti-MeCP2 - 4171289	4171289

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