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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

444905 Sigma-AldrichAnti-Mast Cell Tryptase Mouse mAb (AA1)

This Anti-Mast Cell Tryptase Mouse mAb (AA1) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of Mast Cell Tryptase.

Anti-Mast Cell Tryptase Mouse mAb (AA1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
Ca, Fe, H, Mk	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the 32-38 kDa α- and β-isoforms of tryptase in mast cells.
Catalogue Number	444905
Brand Family	Calbiochem®
Application Data	Detection of human mast cell tryptase by immunohistochemistry. Sample: Human tonsil. Priamry antibody: Anti-Mast Cell Tryptase Mouse mAb (AA1) (Cat. No. 444905) (1:2000). Detection: fluorescence.

References
References	Sommerhoff, C.P., et al. 2000. Biochim. Biophys. Acta.1477, 75. Little, S.S., and Johnson, D.A. 1995. Biochem. J. 307, 341. Morgan, S.J., et al. 1991. J. Allergy Clin. Immunol. 87, 111. Walls, A.F., et al. 1990. J. Pathol. 162, 119. Wall, A.F., et al. 1990. Clin. Exp. Allergy 20, 581.

Product Information
Form	Liquid
Formulation	In 10 mM PBS, pH 7.4.
Positive control	Cat, dog, human, or monkey mast cells
Preservative	None
Quality Level	MQ100

Applications
Key Applications	Frozen Sections Immunoblotting (Western Blotting) Paraffin Sections
Application Notes	Frozen Sections (0.06-0.12 µg/ml) Immunoblotting (1 µg/ml) Paraffin Sections (0.06-0.12 µg/ml, heat pre-treatment required, see comments)
Application Comments	Staining of formalin-fixed paraffin sections requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min. Variables associated with assay conditions will dictate the optimal working dilution.

Biological Information
Immunogen	native, human mast cell, tryptase
Immunogen	Human
Clone	AA1
Host	Mouse
Isotype	IgG₁
Species Reactivity	Canine Feline Human Monkey
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
444905-100UGCN	04055977186208

Documentation

Anti-Mast Cell Tryptase Mouse mAb (AA1) MSDS

职位
物料安全数据表 (MSDS)

Anti-Mast Cell Tryptase Mouse mAb (AA1) 分析证书

标题	批号
444905	输入批号

参考

参考信息概述
Sommerhoff, C.P., et al. 2000. Biochim. Biophys. Acta.1477, 75. Little, S.S., and Johnson, D.A. 1995. Biochem. J. 307, 341. Morgan, S.J., et al. 1991. J. Allergy Clin. Immunol. 87, 111. Walls, A.F., et al. 1990. J. Pathol. 162, 119. Wall, A.F., et al. 1990. Clin. Exp. Allergy 20, 581.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	23-October-2008 JSW
Application	Frozen Sections (0.06-0.12 µg/ml) Immunoblotting (1 µg/ml) Paraffin Sections (0.06-0.12 µg/ml, heat pre-treatment required, see comments)
Application Data	Detection of human mast cell tryptase by immunohistochemistry. Sample: Human tonsil. Priamry antibody: Anti-Mast Cell Tryptase Mouse mAb (AA1) (Cat. No. 444905) (1:2000). Detection: fluorescence.
Description	Protein G purified mouse monoclonal antibody. Recognizes the ~32-38 kDa α- and β-isoforms of tryptase in mast cells.
Background	Mast cells are tissue bound cells that normally are distributed throughout connective tissues. They are involved in inflammatory processes as important effector and regulatory cells. Mast cells are known to accumulate at sites of inflammation, such as asthma, allergic rhinitis, and rheumatoid arthritis. Mast cells contain preformed chemical mediators including histamine, chymase, carboxypeptidase and proteolytic tryptase. Mast cells of the skin and lung contain large amounts of tryptase, suggesting this enzyme has a major role in mast cell-mediated events. Human mast cell tryptase has the potential to be used as marker for mast cell activation and as a mediator of inflammation.
Host	Mouse
Immunogen species	Human
Immunogen	native, human mast cell, tryptase
Clone	AA1
Isotype	IgG₁
Species	canine, feline, human, monkey
Positive control	Cat, dog, human, or monkey mast cells
Form	Liquid
Formulation	In 10 mM PBS, pH 7.4.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	None
Comments	Staining of formalin-fixed paraffin sections requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min. Variables associated with assay conditions will dictate the optimal working dilution.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Standard Handling
References	Sommerhoff, C.P., et al. 2000. Biochim. Biophys. Acta.1477, 75. Little, S.S., and Johnson, D.A. 1995. Biochem. J. 307, 341. Morgan, S.J., et al. 1991. J. Allergy Clin. Immunol. 87, 111. Walls, A.F., et al. 1990. J. Pathol. 162, 119. Wall, A.F., et al. 1990. Clin. Exp. Allergy 20, 581.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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