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444105
Sigma-AldrichAnti-Mannose 6-Phosphate Receptor Mouse mAb (2G11)
This Anti-Mannose 6-Phosphate Receptor Mouse mAb (2G11) is validated for use in Immunoblotting, IF, IHC, IP for the detection of Mannose 6-Phosphate Receptor.
More>>This Anti-Mannose 6-Phosphate Receptor Mouse mAb (2G11) is validated for use in Immunoblotting, IF, IHC, IP for the detection of Mannose 6-Phosphate Receptor. Less<<
MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Immunoblotting (1 µg/ml, non-reducing, see comments) Immunofluorescence (see comments) Immunohistochemistry (2-5 µg/ml) Immunoprecipitation (see comments)
Application Comments
Does not detect MPR in hamster (CHO) cells. Not suitable for immunoblotting using reducing conditions. This antibody has also been reported to work for immunoprecipitation and immunofluorescence. Variables associated with assay conditions will dictate the proper working dilution.
Anti-Mannose 6-Phosphate Receptor Mouse mAb (2G11) 分析证书
标题
批号
444105
参考
参考信息概述
Dintzis, S., et al. 1994. J. Biol. Chem.269, 12159. Dintzis, S., and Pfeffer, S. 1990. EMBO J.9, 77.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
07-August-2007 RFH
Application
Immunoblotting (1 µg/ml, non-reducing, see comments) Immunofluorescence (see comments) Immunohistochemistry (2-5 µg/ml) Immunoprecipitation (see comments)
Description
Purified mouse monoclonal antibody. Recognizes the mannose 6-phosphate receptor protein.
Background
Lysosomal enzymes containing one or two mannose 6-phosphate (man6P) moieties are moved about in the cell by two distinct but interconnected cycles by means of ~300 kDa cation-independent MPRs. The biosynthetic cycle refers to the MPR transport of newly synthesized lysosomal enzymes from the trans Golgi network to late endosomes or early lysosomes. The endocytic cycle transports extracellular lysosomal enzymes from the plasma membrane via clathrin-coated vesicles to early endosomes. The entire pool of MPRs cycles between these cellular compartments every 3 hours. The steady state distribution of MPRs is predominantly late endosomes, fewer in the trans Golgi network, and ~10% at cell surface. In addition to its man6P binding activity, MPR contains a separate binding site for the type II insulin-like growth factor and is capable of binding both man6P and IGF-II simultaneously. An ~240 kDa soluble, truncated form of the extracellular domain of MPR has been detected circulating in serum that is capable of binding both ligands.
Please refer to vial label for lot-specific concentration
Preservative
≤0.1% sodium azide
Comments
Does not detect MPR in hamster (CHO) cells. Not suitable for immunoblotting using reducing conditions. This antibody has also been reported to work for immunoprecipitation and immunofluorescence. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Toxicity
Standard Handling
References
Dintzis, S., et al. 1994. J. Biol. Chem.269, 12159. Dintzis, S., and Pfeffer, S. 1990. EMBO J.9, 77.
