数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

NA27 Sigma-AldrichAnti-MSH2 (Ab-2) Mouse mAb (FE11)

This Anti-MSH2 (Ab-2) Mouse mAb (FE11) is validated for use in Frozen Sections Immunoblotting Immunoprecipitation Paraffin Sections for the detection of MSH2 (Ab-2).

Anti-MSH2 (Ab-2) Mouse mAb (FE11) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

分析证书
参考
小册子
Data Sheet

同义词: Anti-Mismatch Repair Protein 2

Recommended Products

概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
H, M	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the ~100 kDa MSH2 protein in HCT116 and SW480 cells and in colon tissue.
Catalogue Number	NA27
Brand Family	Calbiochem®
Synonyms	Anti-Mismatch Repair Protein 2

References
References	Bronner, C.E., et al. 1994. Nature 368, 258. Papadopoulos, N., et al. 1994. Science 263, 1625. Peltomäki, P.T. 1994. Annals Med. 26, 215. Fishel, R., et al. 1993. Cell 75, 1027. Leach, F.S., et al. 1993. Cell 75, 1215. Lindbolm, A., et al. 1993. Nature Genetics 5, 279.

Product Information
Form	Liquid
Formulation	In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Negative control	LoVo cells
Positive control	HCT116 or SW480 cells or colon tissue
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Application References	Immunoblotting, Frozen Sections Leach, F.S., et al. 1996. Cancer Res. 56, 235. Paraffin Sections Thibodeau, S.N., et al. 1996. Cancer Res. 56, 4836.
Key Applications	Frozen Sections Immunoblotting (Western Blotting) Immunoprecipitation Paraffin Sections
Application Notes	Frozen Sections (2 µg/ml, see application references) Immunoblotting (0.2 µg/ml, see application references) Immunoprecipitation (1 µg/reaction) Paraffin Sections (2 µg/ml, heat or proteinase K pre-treatment required, see application references)
Application Comments	Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	a C-terminal fragment of human MSH2
Immunogen	Human
Clone	FE11
Host	Mouse
Isotype	IgG₁
Species Reactivity	Human Mouse
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Yes

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
NA27-100UGCN	07790788053857

Documentation

Anti-MSH2 (Ab-2) Mouse mAb (FE11) 分析证书

标题	批号
NA27	输入批号

参考

参考信息概述
Bronner, C.E., et al. 1994. Nature 368, 258. Papadopoulos, N., et al. 1994. Science 263, 1625. Peltomäki, P.T. 1994. Annals Med. 26, 215. Fishel, R., et al. 1993. Cell 75, 1027. Leach, F.S., et al. 1993. Cell 75, 1215. Lindbolm, A., et al. 1993. Nature Genetics 5, 279.

小册子

标题
Caspases and other Apoptosis Related Tools Brochure

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	05-September-2007 RFH
Synonyms	Anti-Mismatch Repair Protein 2
Application	Frozen Sections (2 µg/ml, see application references) Immunoblotting (0.2 µg/ml, see application references) Immunoprecipitation (1 µg/reaction) Paraffin Sections (2 µg/ml, heat or proteinase K pre-treatment required, see application references)
Description	Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/O mouse myeloma cells. Recognizes the ~100 kDa MSH2 protein.
Background	The MSH2 gene is one of at least 4 genes encoding proteins involved in the repair of mismatch nucleotides following DNA replication or repair. Mutations in the MSH2 gene contribute to the development of sporadic colorectal carcinoma, while germline MSH2 mutations are responsible for ~50% of inherited, non-polyposis colorectal carcinoma (HNPCC). MSH2 encodes a protein of 934 amino acids.
Host	Mouse
Immunogen species	Human
Immunogen	a C-terminal fragment of human MSH2
Clone	FE11
Isotype	IgG₁
Species	human, mouse
Positive control	HCT116 or SW480 cells or colon tissue
Negative control	LoVo cells
Form	Liquid
Formulation	In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Yes
Toxicity	Standard Handling
References	Bronner, C.E., et al. 1994. Nature 368, 258. Papadopoulos, N., et al. 1994. Science 263, 1625. Peltomäki, P.T. 1994. Annals Med. 26, 215. Fishel, R., et al. 1993. Cell 75, 1027. Leach, F.S., et al. 1993. Cell 75, 1215. Lindbolm, A., et al. 1993. Nature Genetics 5, 279.
Application references	Immunoblotting, Frozen Sections Leach, F.S., et al. 1996. Cancer Res. 56, 235. Paraffin Sections Thibodeau, S.N., et al. 1996. Cancer Res. 56, 4836.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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