Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Recognizes the ~72 kDa latent and the ~66 kDa active forms of MMP-2.
Catalogue Number
IM51
Brand Family
Calbiochem®
Synonyms
Anti-72 kDa Gelatinase, Anti-Gelatinase A, Anti-Matrix Metalloproteinase 2
Application Data
Detection of human MMP-2 by immunoblotting. Sample: Conditioned medium of normal human skin fibroblasts CCD-41SK cells. Primary antibody: Anti-MMP-2 (Ab-4) Mouse mAb (75-7F7) (Cat. No. IM51) (1 µg/ml). Detection: chemiluminescence.
References
References
Cottam, D. W. and Rees, R. C., 1993. Intl. J. Oncol.2, 861. Fujimoto, N., et al. 1993. Clinica Chimica Acta.221, 91. Stetler-Stevenson, W. G., et al. 1993. FASEB J.7, 1434. Woessner, J. F., 1991. FASEB J.5, 2145. Liotta, L. A. and Stetler-Stevenson, W. G., 1990. in Sem. Cancer Biol., ed. M. M. Gottesman. Vol. 1(2), 99.
Product Information
Declaration
Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
Form
Liquid
Formulation
In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
Frozen Sections (see application references) Immunoblotting (1 µg/ml, see application references) Paraffin Sections (see application references)
Application Comments
This antibody does not cross-react with human MMP-1, MMP-3, MMP-7, MMP-8, MMP-9 or MMP-13. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
human MMP-2 derived from conditioned medium of CCD-41SK skin fibroblasts
Immunogen
Human
Clone
75-7F7
Host
Mouse
Isotype
IgG₁
Species Reactivity
Bovine
Human
Mouse
Antibody Type
Monoclonal Antibody
Concentration Label
Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Standard Handling
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号
GTIN
IM51-100UGCN
07790788053802
Documentation
Anti-MMP-2 (Ab-4) Mouse mAb (75-7F7) 分析证书
标题
批号
IM51
参考
参考信息概述
Cottam, D. W. and Rees, R. C., 1993. Intl. J. Oncol.2, 861. Fujimoto, N., et al. 1993. Clinica Chimica Acta.221, 91. Stetler-Stevenson, W. G., et al. 1993. FASEB J.7, 1434. Woessner, J. F., 1991. FASEB J.5, 2145. Liotta, L. A. and Stetler-Stevenson, W. G., 1990. in Sem. Cancer Biol., ed. M. M. Gottesman. Vol. 1(2), 99.
数据表
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
27-August-2007 RFH
Synonyms
Anti-72 kDa Gelatinase, Anti-Gelatinase A, Anti-Matrix Metalloproteinase 2
Application
Frozen Sections (see application references) Immunoblotting (1 µg/ml, see application references) Paraffin Sections (see application references)
Application Data
Detection of human MMP-2 by immunoblotting. Sample: Conditioned medium of normal human skin fibroblasts CCD-41SK cells. Primary antibody: Anti-MMP-2 (Ab-4) Mouse mAb (75-7F7) (Cat. No. IM51) (1 µg/ml). Detection: chemiluminescence.
Description
Purified mouse monoclonal antibody. Recognizes the ~72 kDa latent and the ~66 kDa active forms of MMP-2.
Background
Matrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMPs share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an ~10 kDa segment from the N-terminus and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis.
MMP-2 (Gelatinase A, 72 kDa gelatinase, type IV collagenase, TBE-1) is secreted as a 72 kDa zymogen which is proteolytically processed to the 66 kDa active form. MMP-2, along with its most closely related member of the MMP family, MMP-9, show substrate specificity toward type IV and V collagens, gelatin and elastin. Numerous studies have shown a correlation between collagenase expression and metastatic potential and suggest that it may be a useful marker for the diagnosis or prognosis of cancer.
Host
Mouse
Immunogen species
Human
Immunogen
human MMP-2 derived from conditioned medium of CCD-41SK skin fibroblasts
Clone
75-7F7
Isotype
IgG₁
Species
bovine, human, mouse, not rat
Positive control
MMP-2 protein (Cat. Nos. PF023 or PF037)
Negative control
MMP-9 protein (Cat. Nos. PF024 or PF038)
Form
Liquid
Formulation
In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
Concentration Label
Please refer to vial label for lot-specific concentration
Preservative
≤0.1% sodium azide
Comments
This antibody does not cross-react with human MMP-1, MMP-3, MMP-7, MMP-8, MMP-9 or MMP-13. Antibody should be titrated for optimal results in individual systems.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Toxicity
Standard Handling
References
Cottam, D. W. and Rees, R. C., 1993. Intl. J. Oncol.2, 861. Fujimoto, N., et al. 1993. Clinica Chimica Acta.221, 91. Stetler-Stevenson, W. G., et al. 1993. FASEB J.7, 1434. Woessner, J. F., 1991. FASEB J.5, 2145. Liotta, L. A. and Stetler-Stevenson, W. G., 1990. in Sem. Cancer Biol., ed. M. M. Gottesman. Vol. 1(2), 99.
Application references
Immunoblotting
Fujimoto, N., et al. 1993. Clinica Chimica Acta.221, 91.
Paraffin Sections
Tsunezuka, Y., et al. 1996. Cancer Res.,56, 5678.
Immunohistochemistry
Nakagawa, T., et al. 1994. J. Neurosurg.81, 69.
