数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

ABE1414 Sigma-AldrichAnti-MEIOB Antibody

This Anti-MEIOB Antibody is validated for use in Western Blotting and Immunoprecipitation and Immunocytochemistry for the detection of MEIOB.

Anti-MEIOB Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考

Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: Meiob UniProt Number: Q9D513

View Products on Sigmaaldrich.com

Recommended Products

概述

Replacement Information

特卖

100% Performance Guaranteed

重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
M	WB, IP, ICC	Rb	Serum	Polyclonal Antibody

Description
Catalogue Number	ABE1414
Description	Anti-MEIOB Antibody
Alternate Names	Meiosis-specific with OB domain-containing protein Protein expressed in male leptotene and zygotene spermatocytes 675 MLZ-675
Background Information	MEIOB is a single stranded binding protein required for proper homologous recombination in meiosis I. MEIOB is required for proper DNA strand repair at double strand breaks and faithful synapsis of the chromosomes. MEIOB is also required for successful completion of meiotic recombination and is part of a multiprotein complex with RPA2 and SPATA22 proteins. MEIOB is expressed in the gonads and specifically in ovary and testis starting at the 14th weeks post fertilization. MEIOB is also expressed in a variety of tumors but not in other tissues other than testis and ovary. Because of its unique expression MEIOB is being examined as a possible anti-cancer autoimmune agent.

References

Product Information
Format	Serum
Presentation	Rabbit polyclonal serum with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	This Anti-MEIOB Antibody is validated for use in Western Blotting and Immunoprecipitation and Immunocytochemistry for the detection of MEIOB.
Key Applications	Western Blotting Immunoprecipitation Immunocytochemistry
Application Notes	Immunoprecipitation Analysis: A representative lot immunoprecipitated MEIOB in P18 testes (Luo, M., et al. (2013). Nature. DOI: 10.1038/ncomms3788 pg. 1-12). Immunocytochemistry Analysis: A representative lot detected MEIOB in mouse spermatocytes (Luo, M., et al. (2013). Nature. DOI: 10.1038/ncomms3788 pg. 1-12).

Biological Information
Immunogen	Recombinant protein corresponding to mouse MEIOB.
Concentration	Please refer to lot specific datasheet.
Host	Rabbit
Species Reactivity	Mouse
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_083473
Gene Symbol	Meiob
Purification Method	Unpurified
UniProt Number	Q9D513
Molecular Weight	~50 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in mouse testis tissue lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MEIOB in 10 µg of mouse testis tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
ABE1414	04055977051995

Documentation

Anti-MEIOB Antibody MSDS

职位
物料安全数据表 (MSDS)

Anti-MEIOB Antibody 分析证书

标题	批号
Anti-MEIOB - Q2467698	Q2467698
Anti-MEIOB - 3600645	3600645
Anti-MEIOB Polyclonal Antibody	3014959
Anti-MEIOB Polyclonal Antibody	2935134

参考

参考概述	公共医疗ID
MEIOB exhibits single-stranded DNA-binding and exonuclease activities and is essential for meiotic recombination. Luo, M; Yang, F; Leu, NA; Landaiche, J; Handel, MA; Benavente, R; La Salle, S; Wang, PJ Nature communications 4 2788 2013 显示摘要 Meiotic recombination enables the reciprocal exchange of genetic material between parental homologous chromosomes, and ensures faithful chromosome segregation during meiosis in sexually reproducing organisms. This process relies on the complex interaction of DNA repair factors and many steps remain poorly understood in mammals. Here we report the identification of MEIOB, a meiosis-specific protein, in a proteomics screen for novel meiotic chromatin-associated proteins in mice. MEIOB contains an OB domain with homology to one of the RPA1 OB folds. MEIOB binds to single-stranded DNA and exhibits 3'-5' exonuclease activity. MEIOB forms a complex with RPA and with SPATA22, and these three proteins co-localize in foci that are associated with meiotic chromosomes. Strikingly, chromatin localization and stability of MEIOB depends on SPATA22 and vice versa. Meiob-null mouse mutants exhibit a failure in meiosis and sterility in both sexes. Our results suggest that MEIOB is required for meiotic recombination and chromosomal synapsis.	24240703

参考概述

公共医疗ID

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

此产品已添加到您的购物车。