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Recognizes the ~140 kDa MAP4K4 protein in K-562 cells and human breast cancer tissue.
Catalogue Number
ST1641
Brand Family
Calbiochem®
Synonyms
Anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4
Application Data
Detection of human MAP4K4 by immunoblotitng. Sample: Whole cell lysate from K-562 cells. Primary antibody: Anti-MAP4K4 Mouse mAb (4A5) (Cat. No. ST1641) (1-5 µg/ml). Detection: chemiluminescence. Detection of human MAP4K4 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human breast cancer tissue. Primary antibody: Anti-MAP4K4 Mouse mAb (4A5) (Cat. No. ST1641) (3 µg/ml). Detection: colorimetric (DAB).
ELISA (see comments) Immunoblotting (1-5 µg/ml) Paraffin Sections (3 µg/ml)
Application Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
A recombinant polypeptide corresponding to amino acids of 611-710 human MAP4K4, expressed as a GST fusion protein
Clone
4A5
Host
Mouse
Isotype
IgG₁
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
10-November-2010 RFH
Synonyms
Anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4
Application
ELISA (see comments) Immunoblotting (1-5 µg/ml) Paraffin Sections (3 µg/ml)
Application Data
Detection of human MAP4K4 by immunoblotitng. Sample: Whole cell lysate from K-562 cells. Primary antibody: Anti-MAP4K4 Mouse mAb (4A5) (Cat. No. ST1641) (1-5 µg/ml). Detection: chemiluminescence. Detection of human MAP4K4 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human breast cancer tissue. Primary antibody: Anti-MAP4K4 Mouse mAb (4A5) (Cat. No. ST1641) (3 µg/ml). Detection: colorimetric (DAB).
Description
Purified mouse monoclonal antibody. Recognizes the ~140 kDa MAP4K4 protein.
Background
The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-α signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified.
Host
Mouse
Immunogen
A recombinant polypeptide corresponding to amino acids of 611-710 human MAP4K4, expressed as a GST fusion protein
Clone
4A5
Isotype
IgG₁
Species
human
Positive control
K-562 cells, Human breast cancer tissue
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
