数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

MABE884 Sigma-AldrichAnti-Lin-28B, clone 17A2 Antibody

Anti-Lin-28B, clone 17A2 | MABE884 is an antibody against Lin-28B for use in Western Blotting, Immunocytochemistry, Immunoprecipitation.

Anti-Lin-28B, clone 17A2 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: LIN28B, CSDD2 UniProt Number: Q6ZN17

View Products on Sigmaaldrich.com

Recommended Products

概述

Replacement Information

特卖

100% Performance Guaranteed

重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, ICC, IP	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABE884
Description	Anti-Lin-28B, clone 17A2 Antibody
Alternate Names	Protein lin-28 homolog B Lin-28B
Background Information	Lin28 is a developmentally regulated RNA binding protein (RBP). It’s down regulation is mediated in part by Lin4 and miR-125b, the mammalian micro-RNA homolog of Lin4. It is known to be negatively regulated by retinoic acid and act as a translational enhancer by driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis by increasing the number of initiation events per mRNA molecule. Lin28 is necessary and sufficient for selectively blocking the cleavage processing of pri-let-7 miRNAs in embryonic cells (Viswanathan, 2008). Lin28 is a negative regulator of miRNA biogenesis and may play a role in blocking miRNA-mediated differentiation in stem cells and in certain cancers (Viswanathan, 2008). The mechanism by which miR-125b represses Lin28 in mammalian cells appears to involve reductions not only in translational efficiency, but also in mRNA abundance (Wu, 2005). Lin28 has shown to be essential for skeletal muscle differentiation through the translational upregulation of IGF2 expression. It is known to be expressed in testis, placenta, and embryonic stem (ES) cells.

References

Product Information
Format	Purified
Presentation	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-Lin-28B, clone 17A2 \| MABE884 is an antibody against Lin-28B for use in Western Blotting, Immunocytochemistry, Immunoprecipitation.
Key Applications	Western Blotting Immunocytochemistry Immunoprecipitation
Application Notes	Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Lin28B in 10 µg of NTera2 cell lysate. Immunocytochemistry Analysis: A representative lot detected Lin28B in K-562 cells (Prof. N. Kim, Seoul National University). Immunoprecipitation Analysis: A representative lot immunoprecipitated Lin28B in Huh7 cell lysate (Prof. N. Kim, Seoul National University).

Biological Information
Immunogen	Recombinant protein corresponding to human Lin28B.
Clone	17A2
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Specificity	This antibody reacts to both isoforms.
Isotype	IgG1κ
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001004317
Gene Symbol	LIN28B CSDD2
Purification Method	Protein G Purified
UniProt Number	Q6ZN17
Molecular Weight	~30 kDa observed. Uniprot describes 2 isoforms produced by alternative splicing at ~27 kDa and ~20 kDa.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in HepG2 cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected Lin28B in 10 µg of HepG2 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABE884	04055977125771

Documentation

Anti-Lin-28B, clone 17A2 Antibody MSDS

职位
物料安全数据表 (MSDS)

Anti-Lin-28B, clone 17A2 Antibody 分析证书

标题	批号
Anti-Lin-28B, clone 17A2 -Q2502715	Q2502715

Related Products

产品目录编号

描述

ABE1068

Anti-Lin-28B Antibody

显示价格及库存情况

MABE885

Anti-Lin-28B, clone 15G3 Antibody

显示价格及库存情况

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

此产品已添加到您的购物车。