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MABE893 Sigma-AldrichAnti-LSm1 Antibody, clone 5F3

Anti-LSm1 Antibody, clone 5F3 is an antibody against LSm1 for use in western blotting & IP.

Anti-LSm1 Antibody, clone 5F3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考

Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: Lsm1 UniProt Number: Q8VC85

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
M	WB, IP	R	Purified	Monoclonal Antibody

Description
Catalogue Number	MABE893
Description	Anti-LSm1 Antibody, clone 5F3
Alternate Names	U6 snRNA-associated Sm-like protein LSm1
Background Information	The protein named U6 snRNA-associated Sm-like protein LSm1 or Cancer associated Sm-like, or Small nuclear ribonuclear CaSm and encoded by the human gene LSM1/CASM is a critical binding protein that mediates replication dependent histone mRNA degradation during cell division. Lsm1 is part of the Lsm family of proteins. Lsm proteins are a ubiquitous family of proteins characterized by the Sm-domain. They exist as hexa- or heptameric RNA-binding complexes and carry out RNA-related functions. Lsm1 interacts directly with SLBP a histone stem-loop binding protein critical for histone mRNA processing and expression. Histone mRNAs do not contain introns or polyadenylation signals and are processed by direct endonucleolytic cleavage. Lsm1 binds directly to the 3’-terminal U-tract of U6 snRNA . Lsm1 is localized to the nucleus and is up regulated in many cancers including pancreatic cancer and many tumor cell lines.

References

Product Information
Format	Purified
Presentation	Purified rat monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-LSm1 Antibody, clone 5F3 is an antibody against LSm1 for use in western blotting & IP.
Key Applications	Western Blotting Immunoprecipitation
Application Notes	Western Blotting Analysis: A representative lot from an independent laboratory detected LSm1 in MEF cell lysate (Hoefig, K. P., et al. (2012). Nat Struct Mol Biol. 20(1):73-81.). Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated LSm1 from MEF cell lysate (Hoefig, K. P., et al. (2012). Nat Struct Mol Biol. 20(1):73-81.).

Biological Information
Immunogen	Recombinant protein corresponding to mouse LSm1.
Clone	5F3
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rat
Isotype	IgG1
Species Reactivity	Mouse
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_080308
Gene Symbol	Lsm1
Purification Method	Protein G Purified
UniProt Number	Q8VC85
Molecular Weight	~15 kDa observed

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in L-929 cell lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected LSm1 in 10 µg of L-929 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABE893	04053252946882

Documentation

Anti-LSm1 Antibody, clone 5F3 MSDS

职位
物料安全数据表 (MSDS)

Anti-LSm1 Antibody, clone 5F3 分析证书

标题	批号
Anti-LSm1, clone 5F3 - Q2366891	Q2366891

参考

参考概述	公共医疗ID
Eri1 degrades the stem-loop of oligouridylated histone mRNAs to induce replication-dependent decay. Hoefig, Kai P, et al. Nat. Struct. Mol. Biol., 20: 73-81 (2013) 2013 显示摘要 The exoRNase Eri1 inhibits RNA interference and trims the 5.8S rRNA 3' end. It also binds to the stem-loop of histone mRNAs, but the functional importance of this interaction remains elusive. Histone mRNAs are normally degraded at the end of S phase or after pharmacological inhibition of replication. Both processes are impaired in Eri1-deficient mouse cells, which instead accumulate oligouridylated histone mRNAs. Eri1 trims the mature histone mRNAs by two unpaired nucleotides at the 3' end but stalls close to the double-stranded stem. Upon oligouridylation of the histone mRNA, the Lsm1-7 heteroheptamer recognizes the oligo(U) tail and interacts with Eri1, whose catalytic activity is then able to degrade the stem-loop in a stepwise manner. These data demonstrate how degradation of histone mRNAs is initiated when 3' oligouridylation creates a cis element that enables Eri1 to process the double-stranded stem-loop structure.	23202588

参考概述

公共医疗ID

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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