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MABS489 Sigma-AldrichAnti-LRP-2/Megalin Antibody, clone 10D5.1

Anti-LRP-2/Megalin Antibody, clone 10D5.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Signaling Research Sub-Category: Lipid Signaling Gene Symbol: LRP2, DBS UniProt Number: P98164

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, IH(P)	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABS489
Description	Anti-LRP-2/Megalin Antibody, clone 10D5.1
Alternate Names	Low-density lipoprotein receptor-related protein 2 Calcium sensor protein Glycoprotein 330 gp330 LRP-2 Megalin
Background Information	Low-density lipoprotein receptor-related protein 2 (UniProt P98164; also known as Calcium sensor protein, Glycoprotein 330, gp330, Heymann nephritis antigen homolog, LRP-2, Megalin) is encoded by the LRP2 (also known as DBS) gene (Gene ID 4036) in human. Megalin/LRP-2 is a 600 kDa type I membrane receptor initially produced with a signal peptide sequence (a.a. 1-25). The mature receptor has a large extracellular region (a.a. 26-4423), a transmembrane segment (a.a. 4424-4446), and cytoplasmic tail (a.a. 4447-4655). Megalin belongs to the LDLR family of membrane glycoproteins whose members also inculde the low-density lipoprotein receptor (LDLR), the very-low-density lipoprotein (VLDL) receptor, the LDLR-related protein (LRP), and the apolipoprotein E receptor 2 (ApoER2). The overall extracellular structural features are similar among all LDLR family members. Megalin contains four clusters of cysteine-rich Complement-type/LDLR Class A repeats that mediate ligand-binding. These are separated by 17 EGF-like repeats and eight cysteine-poor spacer regions that contain YWTD motifs involved in the pH-dependent ligands dissociation in endosomal compartments. Megalin is a multiligand, endocytic receptor expressed primarily in the apical membranes of polarized epithelial cells. In addtion to functioning alone, megalin is also reported to interact with another structurally distinct endocytic receptor cubilin in several tissues where they are co-expressed. The internalization of several cubilin ligands is reported to be strongly facilitated in the presence of megalin.

References

Product Information
Format	Purified
Presentation	Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Key Applications	Western Blotting Immunohistochemistry (Paraffin)
Application Notes	Western Blotting Analysis: 0.5 µg/mL from a representative lot detected LRP-2/Megalin in 10 µg of human kidney tissue lysate.

Biological Information
Immunogen	GST-tagged recombinant protein corresponding to the LDL-receptor class A repeats 19-21 of human LRP-2/Megalin.
Epitope	LDL-receptor class A repeats 19-21.
Clone	10D5.1
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Isotype	IgG1κ
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_004516
Gene Symbol	LRP2 DBS
Purification Method	Protein G Purified
UniProt Number	P98164
Molecular Weight	~250 to 600 kDa observed. A broad/smeared banding pattern is consistent with the detection of the target protein with varying degrees of glycosylation (520 kDa/non-glycosylated to 660 kDa/fully glycosylated), as well as smaller size degradation products.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Immunohistochemistry in human kidney tissue. Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected LRP-2/Megalin in human kidney tissue.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 μg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABS489	04055977311754

Documentation

Anti-LRP-2/Megalin Antibody, clone 10D5.1 MSDS

职位
物料安全数据表 (MSDS)

Anti-LRP-2/Megalin Antibody, clone 10D5.1 分析证书

标题	批号
Anti-LRP-2/Megalin, clone 10D5.1 - 3276064	3276064
Anti-LRP-2/Megalin, clone 10D5.1 - 3425385	3425385
Anti-LRP-2/Megalin, clone 10D5.1 - 3880374	3880374
Anti-LRP-2/Megalin, clone 10D5.1 - 3978175	3978175
Anti-LRP-2/Megalin, clone 10D5.1 - 4074796	4074796
Anti-LRP-2/Megalin, clone 10D5.1 - 4206972	4206972
Anti-LRP-2/Megalin, clone 10D5.1 -Q2636947	Q2636947
Anti-LRP-2/Megalin, clone 10D5.1 Monoclonal Antibody	2938796
Anti-LRP-2/Megalin, clone 10D5.1 Monoclonal Antibody	3013240

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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