数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

ST1622 Sigma-AldrichAnti-LEPRE1 Mouse mAb (3C7)

This Anti-LEPRE1 Mouse mAb (3C7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of LEPRE1.

Anti-LEPRE1 Mouse mAb (3C7) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
参考
Data Sheet

同义词: Anti-Leucine Proline-Enriched Proteoglycan 1, Anti-Leprecan 1

View Products on Sigmaaldrich.com

Recommended Products

概述

Replacement Information

重要规格表

Host	Antibody Type
M	Monoclonal Antibody

价格及供货情况

产品目录编号	库存情况	包装	数量 / 包装	价格	数量
ST1622-100UGCN	正在查询供货情况...... — 目前缺货有货停产少量现货供货情况有待确认登录查看库存有货目前缺货有货其余：另行通知其余：另行通知另行通知联系客户服务 Contact Customer Service 联系客户服务联系客户服务		100 μg	正在检索价格...... 无法检索价格。最小订购数量为倍数 Maximum Quantity is 以订单确认为准更多信息您节约了 ()。 — Request Pricing 登录查看价格	查看库存情况 —	添加到收藏夹添加到收藏夹添加到我的收藏夹

Description
Overview	Recognizes the ~90 kDa LEPRE1 protein in HeLa cells and human esophagus tissue.
Catalogue Number	ST1622
Brand Family	Calbiochem®
Synonyms	Anti-Leucine Proline-Enriched Proteoglycan 1, Anti-Leprecan 1
Application Data	Detection of human LEPRE1 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-LEPRE1 Mouse mAb (3C7) (Cat. No. ST1622) (1-5 µg/ml). Detection: chemiluminescence. Detection of human LEPRE1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-LEPRE1 Mouse mAb (3C7) (Cat. No. ST1622) (10 µg/ml). Detection: fluorescence. Detection of human LEPRE1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human esophagus tissue. Primary antibody: Anti-LEPRE1 Mouse mAb (3C7) (Cat. No. ST1622) (6 µg/ml). Detection: colorimetric (DAB).

References
References	Choi, JW, et al. 2009. PLoS Genet. 5, e1000750. Cabral, W,A., et al. 2007. Nat Genet. 39, 359.

Product Information
Form	Liquid
Formulation	In PBS, pH 7.2.
Positive control	HeLa cells, Human esophagus tissue
Preservative	None
Quality Level	MQ100

Applications
Key Applications	Enzyme-Linked Immunosorbent Assay Immunoblotting (Western Blotting) Immunocytochemistry Paraffin Sections
Application Notes	ELISA (see comments) Immunoblotting (1-5 µg/ml) Immunocytochemistry (10 µg/ml) Paraffin Sections (6 µg/ml)
Application Comments	For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Biological Information
Immunogen	A recombinant polypeptide corresponding to amino acids 1-100 of human LEPRE1, expressed as a GST fusion protein
Clone	3C7
Host	Mouse
Isotype	IgG_2a
Antibody Type	Monoclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Regulatory Review
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
ST1622-100UGCN	04055977223699

Documentation

Anti-LEPRE1 Mouse mAb (3C7) MSDS

职位
物料安全数据表 (MSDS)

参考

参考信息概述
Choi, JW, et al. 2009. PLoS Genet. 5, e1000750. Cabral, W,A., et al. 2007. Nat Genet. 39, 359.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	09-November-2010 RFH
Synonyms	Anti-Leucine Proline-Enriched Proteoglycan 1, Anti-Leprecan 1
Application	ELISA (see comments) Immunoblotting (1-5 µg/ml) Immunocytochemistry (10 µg/ml) Paraffin Sections (6 µg/ml)
Application Data	Detection of human LEPRE1 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-LEPRE1 Mouse mAb (3C7) (Cat. No. ST1622) (1-5 µg/ml). Detection: chemiluminescence. Detection of human LEPRE1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-LEPRE1 Mouse mAb (3C7) (Cat. No. ST1622) (10 µg/ml). Detection: fluorescence. Detection of human LEPRE1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human esophagus tissue. Primary antibody: Anti-LEPRE1 Mouse mAb (3C7) (Cat. No. ST1622) (6 µg/ml). Detection: colorimetric (DAB).
Description	Purified mouse monoclonal antibody. Recognizes the ~90 kDa LEPRE1 protein.
Background	This gene encodes an enzyme that is a member of the collagen prolyl hydroxylase family. These enzymes are localized to the endoplasmic reticulum and their activity is required for proper collagen synthesis and assembly. Mutations in this gene are associated with osteogenesis imperfecta type VIII. Two alternatively spliced transcript variants encoding different isoforms have been described. Other variants may exist, but their biological validity has not been determined.
Host	Mouse
Immunogen	A recombinant polypeptide corresponding to amino acids 1-100 of human LEPRE1, expressed as a GST fusion protein
Clone	3C7
Isotype	IgG_2a
Positive control	HeLa cells, Human esophagus tissue
Form	Liquid
Formulation	In PBS, pH 7.2.
Preservative	None
Comments	For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Regulatory Review
References	Choi, JW, et al. 2009. PLoS Genet. 5, e1000750. Cabral, W,A., et al. 2007. Nat Genet. 39, 359.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

此产品已添加到您的购物车。