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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

420400 Sigma-AldrichAnti-KDEL Mouse mAb (10C3)

This Anti-KDEL Mouse mAb (10C3) is validated for use in Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of KDEL.

Anti-KDEL Mouse mAb (10C3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

分析证书
参考
Data Sheet

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
Ca, Dr, Gp, Ht, H, Mk, M, Porcine, Rb, R, Sh, Yeast	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the Grp78 (BiP) and Grp94 proteins.
Catalogue Number	420400
Brand Family	Calbiochem®
Application Data	Detection of KDEL by immunoblotting. Samples: Recombinant Grp78 (BiP) protein (lane 1), whole cell lysate RK-13 cells (lane 2), mouse liver microsomes (lane 3), rat liver microsomes (lane 4), and whole cell lysate from heat shocked HeLa cells (lane 5). Primary antibody: Anti-KDEL Mouse mAb (10C3) (Cat. No. 420400) (1:1000). Detection: chemiluminescence.

References
References	Kuznetsov, G., et al. 1994. J. Biol. Chem. 269, 22990. Huovila, A.-P., et al. 1992. J. Cell Biol. 118, 1305. Munro, S., and Pelham, H.R.B. 1987. Cell 48, 899. Munro, S., and Pelham, H.R.B. 1986. Cell 46, 291.

Product Information
Form	Liquid
Formulation	In PBS, 50% glycerol.
Positive control	RK-13 cells, Mouse liver microsomes, Rat liver microsomes, Heat shocked HeLa cells
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Key Applications	Immunoblotting (Western Blotting) Immunocytochemistry Immunoprecipitation
Application Notes	Immunoblotting (4 µg/ml, colorimetric) Immunocytochemistry (5 µg/ml) Immunoprecipitation (5 µg/ml)
Application Comments	Also recognizes unidentified proteins at ~55 kDa and ~43 kDa by immunoblotting. Does not cross-react with Hsp70. Variables associated with assay conditions will dictate the proper working dilution.

Biological Information
Immunogen	a synthetic peptide (SEKDEL) corresponding to amino acids 649-654 of rat Grp78, conjugated to KLH
Immunogen	Rat
Clone	10C3
Host	Mouse
Isotype	IgG_2a
Species Reactivity	Canine Drosophila Guinea Pig Hamster Human Monkey Mouse Porcine Rabbit Rat Sheep Yeast
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Highly Toxic
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
420400-50UGCN	04055977187571

Documentation

Anti-KDEL Mouse mAb (10C3) 分析证书

标题	批号
420400	输入批号

参考

参考信息概述
Kuznetsov, G., et al. 1994. J. Biol. Chem. 269, 22990. Huovila, A.-P., et al. 1992. J. Cell Biol. 118, 1305. Munro, S., and Pelham, H.R.B. 1987. Cell 48, 899. Munro, S., and Pelham, H.R.B. 1986. Cell 46, 291.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	06-August-2007 RFH
Application	Immunoblotting (4 µg/ml, colorimetric) Immunocytochemistry (5 µg/ml) Immunoprecipitation (5 µg/ml)
Application Data	Detection of KDEL by immunoblotting. Samples: Recombinant Grp78 (BiP) protein (lane 1), whole cell lysate RK-13 cells (lane 2), mouse liver microsomes (lane 3), rat liver microsomes (lane 4), and whole cell lysate from heat shocked HeLa cells (lane 5). Primary antibody: Anti-KDEL Mouse mAb (10C3) (Cat. No. 420400) (1:1000). Detection: chemiluminescence.
Description	Protein G purified mouse monoclonal antibody. Recognizes the KDEL Grp78 (BiP) and Grp94 proteins.
Background	Several resident proteins in the ER are involved in the proper assembly and folding of newly synthesized proteins. These include the glucose-regulated protein 94 (Grp94), BiP, which functions in protein oligomerization, and protein disulfide isomerase (PDI), which catalyzes the rearrangement of the disulfide bond. Grp94, BiP, and PDI share a carboxy-terminal sequence (Lys-Asp-Glu-Leu) with other resident ER proteins. The KDEL sequence is sufficient for the retention of a protein in the ER.
Host	Mouse
Immunogen species	Rat
Immunogen	a synthetic peptide (SEKDEL) corresponding to amino acids 649-654 of rat Grp78, conjugated to KLH
Clone	10C3
Isotype	IgG_2a
Species	Drosophila, canine, guinea pig, hamster, human, monkey, mouse, porcine, rabbit, rat, sheep, yeast
Positive control	RK-13 cells, Mouse liver microsomes, Rat liver microsomes, Heat shocked HeLa cells
Form	Liquid
Formulation	In PBS, 50% glycerol.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	Also recognizes unidentified proteins at ~55 kDa and ~43 kDa by immunoblotting. Does not cross-react with Hsp70. Variables associated with assay conditions will dictate the proper working dilution.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Highly Toxic
References	Kuznetsov, G., et al. 1994. J. Biol. Chem. 269, 22990. Huovila, A.-P., et al. 1992. J. Cell Biol. 118, 1305. Munro, S., and Pelham, H.R.B. 1987. Cell 48, 899. Munro, S., and Pelham, H.R.B. 1986. Cell 46, 291.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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