Millipore Sigma Vibrant Logo

MABF2165-100UL Anti-Influenza A M1/M2 Antibody, clone E10

MABF2165-100UL
100 µL  
无法检索价格。
最小订购数量为倍数
Maximum Quantity is
以订单确认为准 更多信息
您节约了 ()。
 
Request Pricing
目前缺货
目前缺货
有货 
停产
少量现货
供货情况有待确认
    其余:另行通知
      其余:另行通知
      另行通知
      联系客户服务
      Contact Customer Service

       

      联系客户服务

      概述

      Description
      Catalogue NumberMABF2165-100UL
      DescriptionAnti-Influenza A M1/M2 Antibody, clone E10
      Alternate Names
      • M1/M2 protein
      • Proton channel protein M2
      Background InformationMatrix protein 1/2 (UniProt: P03485/P06821; also known as M1/Proton channel protein M2) are encoded by the M gene (Gene ID: 956527/956528) in Influenza A virus (strain A/Puerto Rico/8/1934 H1N1). Influenza A virus is an enveloped virus of the Orthomyxoviridae family with a segmented negative-strand RNA genome. Its M1 protein is a major structural component that forms a coat inside the viral envelope and acts as a bifunctional membrane/RNA-binding protein that mediates the encapsidation of RNA-nucleoprotein cores into the membrane envelope. M2, a single-pass type III, homotetrameric membrane protein, although not essential for infectivity, is required for efficient viral ribonucleoprotein uncoating during viral entry. It forms a proton-selective ion channel that is essential for the efficient release of the viral genome during virus entry. After attaching to the cell surface, the virion enters the cell by endocytosis. Acidification of the endosome is shown to trigger M2 ion channel activity. The influx of protons into virion interior is reported to disrupt interactions between the viral ribonucleoprotein, M1, and lipid bilayers, thereby freeing the viral genome from interaction with viral proteins and enabling RNA segments to migrate to the host cell nucleus, where virus RNA transcription and replication occur. M2 protein is reported to elevate the intravesicular pH of normally acidic compartments, such as trans-Golgi network, and prevents newly formed hemagglutinin from premature switching to the fusion-active conformation. (Ref.: Bourmakina, SV., and Garcı´a-Sastre, A. (2005). J. Virol. 79(12); 7926-7932).
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Influenza A M1/M2, clone E10, Cat. No. MABF2165, is a mouse monoclonal antibody that detects Influenza A virus Matrix protein 1/2 and is tested in Flow Cytometry, Immunocytochemistry, Immunofluorescence, and Western Blotting.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunofluorescence
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Influenza A M1/M2 in Western Blotting applications (Yondola, M.A., et. al. (2011). J Virol. 85(6):2480-91; Bourmakina, S.V., et. al. (2005). J Virol. 79(12):7926-32; Gannage, M., et. al. (2009). Cell Host Microbe. 6(4):367-80).

      Flow Cytometry Analysis: A representative lot detected Influenza A M1/M2 in Flow Cytometry applications (Gannage, M., et. al. (2009). Cell Host Microbe. 6(4):367-80).

      Immunocytochemistry Analysis: A representative lot detected Influenza A M1/M2 in Immunocytochemistry applications (Ramos, I., et. al. (2013). J Virol. 87(5):2430-40).

      Immunofluorescence Analysis: A representative lot detected Influenza A M1/M2 in Immunofluorescence applications (Gannage, M., et. al. (2009). Cell Host Microbe. 6(4):367-80).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 11 amino acids from M2 protein of Influenza A virus that protrudes out of the viral and cellular membrane.
      Epitopeextracellular domain
      CloneE10
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone E10 is a mouse monoclonal antibody that detects both M1 and M2 proteins of Influenza A virus.
      IsotypeIgG2aκ
      Species Reactivity
      • Virus
      Species Reactivity NoteInfluenza A Virus.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • M
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight27.89 and 11.05 kDa calculated for M1 and M2 proteins, respectively. Uncharacterized bands may be observed in some lysate(s).
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MDCK cells infected with Influenza A virus, PR8 strain.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Influenza A virus Matrix protein in lysate from MDCK cells infected with Puerto Rico (PR8) Influenza A virus, but not in lysate from uninfected MDCK cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      产品目录编号 GTIN
      MABF2165-100UL 04061841878315