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Detection of human IMPDH2 by immunoblotting. Sample: Whole cell lysate from 293T cells transfected with full-length, recombinant, human IMPDH2 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-IMPDH2 Mouse mAb (1E12-B6) (Cat. No. ST1578) (1-5 µg/ml). Detection: chemiluminescence.
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
Full-length, recombinant human IMPDH2 (aa 1-515), expressed as a GST fusion protein
Clone
1E12-B6
Host
Mouse
Isotype
IgG₁
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
08-November-2010 RFH
Synonyms
Anti-Inosine Monophosphate Dehydrogenase 2
Application
ELISA (see comments) Immunoblotting (1-5 µg/ml)
Application Data
Detection of human IMPDH2 by immunoblotting. Sample: Whole cell lysate from 293T cells transfected with full-length, recombinant, human IMPDH2 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-IMPDH2 Mouse mAb (1E12-B6) (Cat. No. ST1578) (1-5 µg/ml). Detection: chemiluminescence.
Description
Purified mouse monoclonal antibody. Recognizes the IMPDH2 protein.
Background
IMPDH2 is a rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. This protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate.
Host
Mouse
Immunogen
Full-length, recombinant human IMPDH2 (aa 1-515), expressed as a GST fusion protein
Clone
1E12-B6
Isotype
IgG₁
Species
human
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
