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Recognizes the ~50 kDa IL13RA2 protein in HeLa cells.
Catalogue Number
AP1145
Brand Family
Calbiochem®
Synonyms
Anti-Interleukin-13 Receptor αChain 2
Application Data
Detection of human IL13RA2 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-IL13RA2 Mouse mAb (2E10) (Cat. No. AP1145) (1-5 µg/ml). Detection: chemiluminesence.
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
A recombinant polypeptide corresponding to amino acids 27-127 of human IL13RA2, expressed as a GST fusion protein
Clone
2E10
Host
Mouse
Isotype
IgG2a
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
27-October-2010 JLN
Synonyms
Anti-Interleukin-13 Receptor αChain 2
Application
ELISA (see comments) Immunoblotting (1-5 µg/ml)
Application Data
Detection of human IL13RA2 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-IL13RA2 Mouse mAb (2E10) (Cat. No. AP1145) (1-5 µg/ml). Detection: chemiluminesence.
Description
Purified mouse monoclonal antibody. Recognizes the ~50 kDa IL13RA2 protein.
Background
IL13RA2 is closely related to IL13RA1, a subunit of the interleukin 13 receptor complex. This protein binds IL13 with high affinity, but lacks a cytoplasmic domain, and does not appear to function as a signal mediator. It is reported to play a role in the internalization of IL13.
Host
Mouse
Immunogen
A recombinant polypeptide corresponding to amino acids 27-127 of human IL13RA2, expressed as a GST fusion protein
Clone
2E10
Isotype
IgG2a
Species
human
Positive control
HeLa cells
Form
Liquid
Formulation
In PBS, pH 7.2.
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
