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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

GR11L Sigma-AldrichAnti-IGF-IR (Ab-1) Mouse mAb (αIR3)

This Anti-IGF-IR (Ab-1) Mouse mAb (αIR3) is validated for use in IF, IP, Neutralization Studies, Paraffin Sections, Immunoblotting for the detection of IGF-IR (Ab-1).

Anti-IGF-IR (Ab-1) Mouse mAb (αIR3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet
Citations

同义词: Anti-IGF-IR, Anti-Insulin-Like Growth Factor Receptor

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
H	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the ~130 kDa α and the ~90 kDa β subunits of IGF-I receptor.
Catalogue Number	GR11L
Brand Family	Calbiochem®
Synonyms	Anti-IGF-IR, Anti-Insulin-Like Growth Factor Receptor

References
References	Roth, R. 1988. Science 239, 1269. Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm. 149, 276. Rosen, O.M. 1987. Science 257, 1452. Rechler, M.M., and Nissley, S.P. 1986. Hormone Res. 24, 152. Ullrich, A., et al. 1986. EMBO J. 5, 2503. Zapf, S., and Froesch, E.R. 1986. Hormone Res. 24, 121. Humbel, R.E. 1984. I. Chemistry in Li Hormonal proteins and peptides. Vol 12, Chap. 4 (Academic Press, New York, 1984). Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561.

References

Roth, R. 1988. Science 239, 1269.
Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm. 149, 276.
Rosen, O.M. 1987. Science 257, 1452.
Rechler, M.M., and Nissley, S.P. 1986. Hormone Res. 24, 152.
Ullrich, A., et al. 1986. EMBO J. 5, 2503.
Zapf, S., and Froesch, E.R. 1986. Hormone Res. 24, 121.
Humbel, R.E. 1984. I. Chemistry in Li Hormonal proteins and peptides. Vol 12, Chap. 4 (Academic Press, New York, 1984).
Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561.

Product Information
Form	Lyophilized
Formulation	Lyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA .
Negative control	HS27 cells
Positive control	HepG2 cells
Preservative	None
Quality Level	MQ100

Applications
Application References	Neutralization Studies Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm. 149, 276. Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561. Original Clone Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561.
Key Applications	Immunofluorescence Immunoprecipitation Not Western Blot Not Paraffin Sections Neutralization Studies
Application Notes	Immunofluorescence (1-5 µg/ml) Immunoprecipitation (1 µg/sample) Neutralization Studies (1 µg/ml; see application references) Paraffin Sections (not recommended) Immunoblotting (not recommended)
Application Comments	Blocks IGF-I binding to the IGF-I receptor. May also weakly cross-react with the insulin receptor (see application references). It also inhibits the growth of MCF-7 cells in culture suggesting the IGF-I receptor may be involved in autocrine regulation of cell growth (see application references). Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	partially purified, human placenta IGF-I receptor
Immunogen	Human
Clone	αIR3
Host	Mouse
Isotype	IgG₁
Species Reactivity	Human
Antibody Type	Monoclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Ok to freeze
Special Instructions	Reconstitute with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least two h. Following reconstitution, aliquot and freeze (-20°C) or refrigerate (4°C) with 0.1% sodium azide. Freezing of aliquots is recommended for long term storage of reconstituted product. Avoid freeze/thaw cycles of solutions.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
GR11L-100UGCN	07790788053758

Documentation

Anti-IGF-IR (Ab-1) Mouse mAb (αIR3) MSDS

职位
物料安全数据表 (MSDS)

Anti-IGF-IR (Ab-1) Mouse mAb (αIR3) 分析证书

标题	批号
GR11L	输入批号

参考

参考信息概述
Roth, R. 1988. Science 239, 1269. Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm. 149, 276. Rosen, O.M. 1987. Science 257, 1452. Rechler, M.M., and Nissley, S.P. 1986. Hormone Res. 24, 152. Ullrich, A., et al. 1986. EMBO J. 5, 2503. Zapf, S., and Froesch, E.R. 1986. Hormone Res. 24, 121. Humbel, R.E. 1984. I. Chemistry in Li Hormonal proteins and peptides. Vol 12, Chap. 4 (Academic Press, New York, 1984). Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561.

参考信息概述

引用

标题

Rita Nahta, et al. (2005) Insulin-like growth factor-I receptor/human epidermal growth factor receptor 2 heterodimerization contributes to trastuzumab resistance of breast cancer cells. Cancer Research 65, 11118-11128.

Daniel E. Syroid, et al. (1999) A role for insulin-like growth factor-I in the regulation of Schwann cell survival. Journal of Neuroscience 19, 2059-2068.

Donald M. Henricks, et al. (1998) Identification of insulin-like growth factor I in bovine seminal plasma and its receptor on spermatozoa: influence on sperm motility. Biology of Reproduction 59, 330-337.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	24-August-2007 RFH
Synonyms	Anti-IGF-IR, Anti-Insulin-Like Growth Factor Receptor
Application	Immunofluorescence (1-5 µg/ml) Immunoprecipitation (1 µg/sample) Neutralization Studies (1 µg/ml; see application references) Paraffin Sections (not recommended) Immunoblotting (not recommended)
Description	Purified mouse monoclonal antibody generated by immunizing SJL mice with the specified immunogen and fusing splenocytes with FO mouse myeloma cells (see application references). Recognizes the ~130 kDa α and the ~90 kDa β subunits of IGF-I receptor.
Background	The insulin-like growth factors (IGFs) or somatomedins are mitogenic peptides present in the plasma of human, rat, and other species. Two distinct but chemically related IGFs have been purified from human plasma: IGF-I (Somatomedin C) and IGF-II. They are single chain polypeptides with molecular weights of 7,640 and 7,471, respectively. Types I and II IGF receptors differ in their structure and in their peptide binding specificity. Type I IGF receptors are structurally homologous to insulin receptors and contain a-subunits (MW = 130,000) that bind peptide, and b-subunits (MW = 90,000), which have intrinsic tyrosine kinase activity. The protein-tyrosine kinase activity of the insulin receptor and, presumably the IGF-I receptor, is required for insulin to stimulate all of its biological responses in cells. Type II receptors are a single polypeptide (MW = 260,000), lack protein-tyrosine kinase activity, and have been shown to be identical to the mannose-6-phosphate receptor.
Host	Mouse
Immunogen species	Human
Immunogen	partially purified, human placenta IGF-I receptor
Clone	αIR3
Isotype	IgG₁
Species	human
Positive control	HepG2 cells
Negative control	HS27 cells
Form	Lyophilized
Formulation	Lyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA .
Preservative	None
Comments	Blocks IGF-I binding to the IGF-I receptor. May also weakly cross-react with the insulin receptor (see application references). It also inhibits the growth of MCF-7 cells in culture suggesting the IGF-I receptor may be involved in autocrine regulation of cell growth (see application references). Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Ok to freeze
Special Instructions	Reconstitute with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least two h. Following reconstitution, aliquot and freeze (-20°C) or refrigerate (4°C) with 0.1% sodium azide. Freezing of aliquots is recommended for long term storage of reconstituted product. Avoid freeze/thaw cycles of solutions.
Toxicity	Standard Handling
References	Roth, R. 1988. Science 239, 1269. Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm. 149, 276. Rosen, O.M. 1987. Science 257, 1452. Rechler, M.M., and Nissley, S.P. 1986. Hormone Res. 24, 152. Ullrich, A., et al. 1986. EMBO J. 5, 2503. Zapf, S., and Froesch, E.R. 1986. Hormone Res. 24, 121. Humbel, R.E. 1984. I. Chemistry in Li Hormonal proteins and peptides. Vol 12, Chap. 4 (Academic Press, New York, 1984). Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561.
Citation	Rita Nahta, et al. (2005) Insulin-like growth factor-I receptor/human epidermal growth factor receptor 2 heterodimerization contributes to trastuzumab resistance of breast cancer cells. Cancer Research 65, 11118-11128. Daniel E. Syroid, et al. (1999) A role for insulin-like growth factor-I in the regulation of Schwann cell survival. Journal of Neuroscience 19, 2059-2068. Donald M. Henricks, et al. (1998) Identification of insulin-like growth factor I in bovine seminal plasma and its receptor on spermatozoa: influence on sperm motility. Biology of Reproduction 59, 330-337.
Application references	Neutralization Studies Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm. 149, 276. Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561. Original Clone Kull, F.C., et al.1983. J. Biol. Chem. 258, 6561.

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GR11

Anti-IGF-IR (Ab-1) Mouse mAb (αIR3)

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Life Science Research > Antibodies and Assays > Primary Antibodies

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