数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

386040 Sigma-AldrichAnti-Hsp90 Mouse mAb (AC88)

This Anti-Hsp90 Mouse mAb (AC88) is validated for use in Immunoblotting, Immunoprecipitation, Immunocytochemistry for the detection of Hsp90.

Anti-Hsp90 Mouse mAb (AC88) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

同义词: Anti-Hsp82, Anti-Hsp85, Anti-Hsp89

View Products on Sigmaaldrich.com

Recommended Products

概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
A Broad Range Of Species	M	Monoclonal Antibody

价格及供货情况

产品目录编号	库存情况	包装	数量 / 包装	价格	数量
386040-50UGCN	正在查询供货情况...... — 目前缺货有货停产少量现货供货情况有待确认登录查看库存有货目前缺货有货其余：另行通知其余：另行通知另行通知联系客户服务 Contact Customer Service 联系客户服务联系客户服务	塑胶安瓿;塑胶针药瓶	50 μg	正在检索价格...... 无法检索价格。最小订购数量为倍数 Maximum Quantity is 以订单确认为准更多信息您节约了 ()。 — Request Pricing 登录查看价格	查看库存情况 —	添加到收藏夹添加到收藏夹添加到我的收藏夹

Description
Overview	Recognizes the ~90 kDa Hsp90 protein.
Catalogue Number	386040
Brand Family	Calbiochem®
Synonyms	Anti-Hsp82, Anti-Hsp85, Anti-Hsp89
Application Data	Detection of Hsp90 by immunoblotting. Samples: Native human Hsp90 protein (lane 1), recombinant human Hsp90β protein (lane 2), recombinant human Hsp90α protein (lane 3), whole cell lysate from heat shocked HeLa cells (lane 4), heat shocked 3T3 cells (lane 5), heat shocked PC-12 cells (lane 6), heat shocked CHO-K1 cells (lane 7), and heat shocked Rat-2 cells (lane 8). Primary antibody: Anti-Hsp90 Mouse mAb (AC88) (Cat. No. 386040) (1:1000). Detection: chemiluminescence.

References
References	Harry, J.L. et al. 1990. Development 190, 305. Minoo, P. et al. 1989 J. Cell. Biol. 109, 1937. Riehl, R.M., et al. 1985. Biochemistry 24, 6586. Sanchez, E.R., et al. 1985. J. Biol. Chem. 260, 12398.

Product Information
Form	Liquid
Formulation	In 10 mM PBS, 50% glycerol, pH 7.2.
Positive control	Heat shocked HeLa cells, Heat shocked 3T3 cells, Heat shocked PC-12 cells, Heat shocked CHO-K1, Heat shocked Rat-2 cells
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Key Applications	Immunoblotting (Western Blotting) Immunocytochemistry Immunoprecipitation
Application Notes	Immunoblotting (1 µg/ml, colorimetric) Immunoprecipitation (see comments) Immunocytochemistry (see comments)
Application Comments	Cross-reacts with glucocorticoid receptor in rodent. By immunoprecipitation, the antibody favors free Hsp90 over complexed Hsp90. The optimal dilution for immunoprecipitation is assay dependent and must be determined for each sample. This antibody has also been reported to work for immunocytochemistry. Variables associated with assay conditions will dictate the optimal dilution.

Biological Information
Immunogen	Hsp90 isolated from water mold, Achyla ambisexualis
Immunogen	Achyla ambisexualis
Clone	AC88
Host	Mouse
Isotype	IgG₁
Species Reactivity	A Broad Range Of Species
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
386040-50UGCN	04055977189896

Documentation

Anti-Hsp90 Mouse mAb (AC88) MSDS

职位
物料安全数据表 (MSDS)

Anti-Hsp90 Mouse mAb (AC88) 分析证书

标题	批号
386040	输入批号

参考

参考信息概述
Harry, J.L. et al. 1990. Development 190, 305. Minoo, P. et al. 1989 J. Cell. Biol. 109, 1937. Riehl, R.M., et al. 1985. Biochemistry 24, 6586. Sanchez, E.R., et al. 1985. J. Biol. Chem. 260, 12398.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	02-August-2007 RFH
Synonyms	Anti-Hsp82, Anti-Hsp85, Anti-Hsp89
Application	Immunoblotting (1 µg/ml, colorimetric) Immunoprecipitation (see comments) Immunocytochemistry (see comments)
Application Data	Detection of Hsp90 by immunoblotting. Samples: Native human Hsp90 protein (lane 1), recombinant human Hsp90β protein (lane 2), recombinant human Hsp90α protein (lane 3), whole cell lysate from heat shocked HeLa cells (lane 4), heat shocked 3T3 cells (lane 5), heat shocked PC-12 cells (lane 6), heat shocked CHO-K1 cells (lane 7), and heat shocked Rat-2 cells (lane 8). Primary antibody: Anti-Hsp90 Mouse mAb (AC88) (Cat. No. 386040) (1:1000). Detection: chemiluminescence.
Description	Protein G purified mouse monoclonal antibody. Recognizes the ~90 kDa Hsp90 protein.
Background	Hsp90 (heat shock protein 90) is a primarily cytoplasmic protein which exists in dimeric form. Hsp90 binds several cellular proteins such as retrovirus kinases (pp60^v-src), steroid receptors, heme-regulated protein kinase, actin, and tubulin. Although Hsp90 expression is induced by stress, and thus may be an indicator of cell stress, its constitutive level may be up to 1-2% of the total cellular protein.
Host	Mouse
Immunogen species	Achyla ambisexualis
Immunogen	Hsp90 isolated from water mold, Achyla ambisexualis
Clone	AC88
Isotype	IgG₁
Species	a broad range of species
Positive control	Heat shocked HeLa cells, Heat shocked 3T3 cells, Heat shocked PC-12 cells, Heat shocked CHO-K1, Heat shocked Rat-2 cells
Form	Liquid
Formulation	In 10 mM PBS, 50% glycerol, pH 7.2.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	Cross-reacts with glucocorticoid receptor in rodent. By immunoprecipitation, the antibody favors free Hsp90 over complexed Hsp90. The optimal dilution for immunoprecipitation is assay dependent and must be determined for each sample. This antibody has also been reported to work for immunocytochemistry. Variables associated with assay conditions will dictate the optimal dilution.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Standard Handling
References	Harry, J.L. et al. 1990. Development 190, 305. Minoo, P. et al. 1989 J. Cell. Biol. 109, 1937. Riehl, R.M., et al. 1985. Biochemistry 24, 6586. Sanchez, E.R., et al. 1985. J. Biol. Chem. 260, 12398.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

此产品已添加到您的购物车。