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			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

386023 Sigma-AldrichAnti-Hsp47 Mouse mAb (M16.10A1)

Anti-Hsp47, mouse monoclonal, clone M16.10A1, recognizes the ~47 kDa Hsp47 protein in wide variety of species. It is validated for use in ELISA, EIA, WB, ICC, IF, IP, and IHC (paraffin sections).

Anti-Hsp47 Mouse mAb (M16.10A1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

同义词: Anti-Colligin

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
B, Ca, Ch, Gp, Ht, H, Mk, M, Porcine, R, Sh	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the ~47 kDa Hsp47 protein.
Catalogue Number	386023
Brand Family	Calbiochem®
Synonyms	Anti-Colligin
Application Data	Detection of mouse Hsp47 by immunoblotting. Sample: Whole cell lysate from 3T3 cells. Primary antibody: Anti-Hsp47 Mouse mAb (M16.10A1) (Cat. No. 386023) (1:1000). Detection: chemiluminescence. (left) Detection of rat Hsp47 by staining paraffin sections. Sample: Paraffin-embedded rat carotoid artery after balloon injury to the artery. Primary antibody: Anti-Hsp47 Mouse mAb (M16.10A1) (Cat. No. 386023). Detection: colorimetric (DAB). (right) Detection of human Hsp47 by staining paraffin sections. Sample: Human breast cancer tissue. Primary antibody: Anti-Hsp47 Mouse mAb (M16.10A1) (Cat. No. 386023) (1:50). Detection: colorimetric (DAB).

References
References	Kuroda, K., et al. 1998. J. Invest. Dermatol. 111, 1023. Nandan, D., et al. 1998. Exp. Cell Res. 179, 289. Clark, E.P., et al. 1991. J. Biol. Chem. 266, 17230. Vaillancourt, J.P., and Cates, G.A. 1991. Biochem. J. 274, 793. Cates, G.A., et al. 1987. Biochem. Cell Biol. 65, 767. Saga, S., et al. 1987. J. Cell Biol. 105, 517. Nagata, K., et al. 1986. J. Biol. Chem. 261, 7531.

References

Kuroda, K., et al. 1998. J. Invest. Dermatol. 111, 1023.
Nandan, D., et al. 1998. Exp. Cell Res. 179, 289.
Clark, E.P., et al. 1991. J. Biol. Chem. 266, 17230.
Vaillancourt, J.P., and Cates, G.A. 1991. Biochem. J. 274, 793.
Cates, G.A., et al. 1987. Biochem. Cell Biol. 65, 767.
Saga, S., et al. 1987. J. Cell Biol. 105, 517.
Nagata, K., et al. 1986. J. Biol. Chem. 261, 7531.

Product Information
Form	Liquid
Formulation	In PBS, 50% glycerol, pH 7.2.
Positive control	3T3 cells, Human breast cancer tissue
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Key Applications	Enzyme Immunoassay Enzyme-Linked Immunosorbent Assay Immunoblotting (Western Blotting) Immunocytochemistry Immunofluorescence Immunoprecipitation Paraffin Sections
Application Notes	ELISA (see comments) Enzyme Immunoassay (see comments) Immunoblotting (1 µg/ml, colorimetric) Immunocytochemistry (1 µg/ml) Immunofluorescence (5 µg/ml) Immunoprecipitation (5 µg/ml) Paraffin Sections (see comments)
Application Comments	This antibody has also been reported to work for ELISA and enzyme immunoassay. Variables associated with assay conditions will dictate the optimal dilution.

Biological Information
Immunogen	Hsp47 from L6 cells
Immunogen	Rat
Clone	M16.10A1
Host	Mouse
Isotype	IgG_2b
Species Reactivity	Bovine Canine Chicken Guinea Pig Hamster Human Monkey Mouse Porcine Rat Sheep
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
386023-50UGCN	04055977189872

Documentation

Anti-Hsp47 Mouse mAb (M16.10A1) MSDS

职位
物料安全数据表 (MSDS)

Anti-Hsp47 Mouse mAb (M16.10A1) 分析证书

标题	批号
386023	输入批号

参考

参考信息概述
Kuroda, K., et al. 1998. J. Invest. Dermatol. 111, 1023. Nandan, D., et al. 1998. Exp. Cell Res. 179, 289. Clark, E.P., et al. 1991. J. Biol. Chem. 266, 17230. Vaillancourt, J.P., and Cates, G.A. 1991. Biochem. J. 274, 793. Cates, G.A., et al. 1987. Biochem. Cell Biol. 65, 767. Saga, S., et al. 1987. J. Cell Biol. 105, 517. Nagata, K., et al. 1986. J. Biol. Chem. 261, 7531.

参考信息概述

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	02-August-2007 RFH
Synonyms	Anti-Colligin
Application	ELISA (see comments) Enzyme Immunoassay (see comments) Immunoblotting (1 µg/ml, colorimetric) Immunocytochemistry (1 µg/ml) Immunofluorescence (5 µg/ml) Immunoprecipitation (5 µg/ml) Paraffin Sections (see comments)
Application Data	Detection of mouse Hsp47 by immunoblotting. Sample: Whole cell lysate from 3T3 cells. Primary antibody: Anti-Hsp47 Mouse mAb (M16.10A1) (Cat. No. 386023) (1:1000). Detection: chemiluminescence. (left) Detection of rat Hsp47 by staining paraffin sections. Sample: Paraffin-embedded rat carotoid artery after balloon injury to the artery. Primary antibody: Anti-Hsp47 Mouse mAb (M16.10A1) (Cat. No. 386023). Detection: colorimetric (DAB). (right) Detection of human Hsp47 by staining paraffin sections. Sample: Human breast cancer tissue. Primary antibody: Anti-Hsp47 Mouse mAb (M16.10A1) (Cat. No. 386023) (1:50). Detection: colorimetric (DAB).
Description	Protein G purified mouse monoclonal antibody. Recognizes the ~47 kDa Hsp47 protein.
Background	Hsp47 is a unique heat shock protein with collagen binding ability. A member of the serpin family of proteins, Hsp47 localizes within the ER due to an RDEL sequence, a variant of the KDEL sequence. Hsp47 is thought to bind procollagen immediately after it co-translationally enters the ER, and it dissociates from procollagen before its secretion. The ability of Hsp47 to bind collagen I and collagen IV suggests it may function as a molecular chaperone for these proteins.
Host	Mouse
Immunogen species	Rat
Immunogen	Hsp47 from L6 cells
Clone	M16.10A1
Isotype	IgG_2b
Species	bovine, canine, chicken, guinea pig, hamster, human, monkey, mouse, porcine, rat, sheep
Positive control	3T3 cells, Human breast cancer tissue
Form	Liquid
Formulation	In PBS, 50% glycerol, pH 7.2.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	This antibody has also been reported to work for ELISA and enzyme immunoassay. Variables associated with assay conditions will dictate the optimal dilution.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Standard Handling
References	Kuroda, K., et al. 1998. J. Invest. Dermatol. 111, 1023. Nandan, D., et al. 1998. Exp. Cell Res. 179, 289. Clark, E.P., et al. 1991. J. Biol. Chem. 266, 17230. Vaillancourt, J.P., and Cates, G.A. 1991. Biochem. J. 274, 793. Cates, G.A., et al. 1987. Biochem. Cell Biol. 65, 767. Saga, S., et al. 1987. J. Cell Biol. 105, 517. Nagata, K., et al. 1986. J. Biol. Chem. 261, 7531.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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