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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

374087 Sigma-AldrichAnti-Heme Oxygenase-1 Mouse mAb (HO-1-1)

Anti-Heme Oxygenase-1, mouse monoclonal, clone HO-1-1, recognizes the ~32 kDa HO-1 in a variety of species. It is validated for use in Western blotting, immunocytochemistry, and immunoprecipitation.

Anti-Heme Oxygenase-1 Mouse mAb (HO-1-1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

同义词: Anti-HO-1, Anti-Hsp32

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
B, Ca, H, Mk, M, R	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the ~32 kDa HO-1 protein.
Catalogue Number	374087
Brand Family	Calbiochem®
Synonyms	Anti-HO-1, Anti-Hsp32
Application Data	Detection of human heme oxygenase-1 by immunoblotting. Sample: Extract from human microsomes. Primary antibody: Anti-Heme Oxygenase-1 Mouse mAb (HO-1-1) (Cat. No. 374087) (1:250). Detection: chemiluminescence. Detection of human heme oxygenase-1 by flow cytometry. Sample: Human lung cancer A2 cells (both panels). Primary antibodies: Isotope control (left panel) and Anti-Heme Oxygenase-1 Mouse mAb (HO-1-1) (right panel) (Cat. No. 374087) (10 µg/ml). Detection: fluorescence.

References
References	Yoshida, T., et al. 1988. Eur. J. Biochem. 171, 457. Maines, M.D. 1988. FASEB J. 2, 2557. Trakshel, G.M., et al. 1986 J. Biol. Chem. 261, 11131.

Product Information
Form	Liquid
Formulation	In PBS, 50% glycerol.
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Key Applications	Immunoblotting (Western Blotting) Immunocytochemistry Immunoprecipitation
Application Notes	Immunoblotting (4 µg/ml, chemiluminescence) Immunocytochemistry (1:1000) Immunoprecipitation (20 µg/ml)
Application Comments	Does not cross-react with HO-2. Variables associated with assay conditions will dictate the proper working dilution.

Biological Information
Immunogen	a synthetic peptide (MERPQPDSMPQDLSEALKEATKEVHTQAEN) corresponding to amino acids 1-30 of human HO-1 prepared as a four-branched multiple antigen peptide
Immunogen	Human
Clone	HO-1-1
Host	Mouse
Isotype	IgG₁
Species Reactivity	Bovine Canine Human Monkey Mouse Rat
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
374087-200UGCN	04055977191165

Documentation

Anti-Heme Oxygenase-1 Mouse mAb (HO-1-1) MSDS

职位
物料安全数据表 (MSDS)

Anti-Heme Oxygenase-1 Mouse mAb (HO-1-1) 分析证书

标题	批号
374087	输入批号

参考

参考信息概述
Yoshida, T., et al. 1988. Eur. J. Biochem. 171, 457. Maines, M.D. 1988. FASEB J. 2, 2557. Trakshel, G.M., et al. 1986 J. Biol. Chem. 261, 11131.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	11-November-2011 JSW
Synonyms	Anti-HO-1, Anti-Hsp32
Application	Immunoblotting (4 µg/ml, chemiluminescence) Immunocytochemistry (1:1000) Immunoprecipitation (20 µg/ml)
Application Data	Detection of human heme oxygenase-1 by immunoblotting. Sample: Extract from human microsomes. Primary antibody: Anti-Heme Oxygenase-1 Mouse mAb (HO-1-1) (Cat. No. 374087) (1:250). Detection: chemiluminescence. Detection of human heme oxygenase-1 by flow cytometry. Sample: Human lung cancer A2 cells (both panels). Primary antibodies: Isotope control (left panel) and Anti-Heme Oxygenase-1 Mouse mAb (HO-1-1) (right panel) (Cat. No. 374087) (10 µg/ml). Detection: fluorescence.
Description	Protein G purified mouse monoclonal antibody. Recognizes the ~32 kDa heme oxygenase (HO-1) protein.
Background	Heme oxygenase (HO) is a microsomal enzyme that oxidatively cleaves heme, a pro-oxidant, into carbon monoxide and biliverdin, which is reduced to the antioxidant, bilirubin. Two isozymes, termed HO-1 and HO-2, have been identified in rat, rabbit, monkey, and human tissues. By SDS-PAGE, mammalian HO-1 is ~31-33 kDa; HO-2 is ~36 kDa. HO-1, also referred to as stress protein Hsp32, is the inducible form of the enzyme. Expression of HO-1 can be induced by a variety of stimuli such as heme, metals, hormones, UV radiation and sulfhydryl depleting agents. In contrast, HO-2 is the constitutive form of the enzyme. HO-2 is the most prevalent form in most tissues, except the spleen where HO-1 levels are predominant.
Host	Mouse
Immunogen species	Human
Immunogen	a synthetic peptide (MERPQPDSMPQDLSEALKEATKEVHTQAEN) corresponding to amino acids 1-30 of human HO-1 prepared as a four-branched multiple antigen peptide
Clone	HO-1-1
Isotype	IgG₁
Species	bovine, canine, human, monkey, mouse, rat
Form	Liquid
Formulation	In PBS, 50% glycerol.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	Does not cross-react with HO-2. Variables associated with assay conditions will dictate the proper working dilution.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Standard Handling
References	Yoshida, T., et al. 1988. Eur. J. Biochem. 171, 457. Maines, M.D. 1988. FASEB J. 2, 2557. Trakshel, G.M., et al. 1986 J. Biol. Chem. 261, 11131.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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