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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

374090 Sigma-AldrichAnti-Heme Oxygenase-1 (1-30) Rabbit pAb

Anti-Heme Oxygenase-1 (1-30), rabbit polyclonal, recognizes the ~32 kDa HO-1 protein. Does not cross-react with HO-2. It is validated for use in Western blotting and immunoprecipitation.

Anti-Heme Oxygenase-1 (1-30) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书
参考
Data Sheet

同义词: Anti-HO-1, Anti-Hsp32

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概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
Ca, Ht, H, Mk, M, R	Rb	Polyclonal Antibody

价格及供货情况

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Description
Overview	Recognizes the ~32 kDa HO-1 protein. Does not cross-react with HO-2.
Catalogue Number	374090
Brand Family	Calbiochem®
Synonyms	Anti-HO-1, Anti-Hsp32
Application Data	Detection of heme oxygenase-1 by immunoblotting. Samples: Recombinant rat HO-1 (lane 1), recombinant human HO-1 (lane 2), negative control recombinant human protein (lane 3), extracts from dog liver microsomes (lane 4), mouse liver microsomes (lane 5), rat liver microsomes (lane 6), and human liver microsomes (lane 7). Primary antibody: Anti-Heme Oxygenase-1 (1-30) Rabbit pAb (Cat. No. 374090) (1:1000). Detection: chemiluminescence.

References
References	Maines, M.D. 1988 FASEB J. 2, 2557. Kutty, R.K., et al. 1994. J. Cell Physiol. 159, 371. Yoshida, T., et al. 1988. Eur. J. Biochem. 171, 457. Trakshel, G.M., et al. 1986 J. Biol. Chem. 261, 11131.

Product Information
Form	Liquid
Formulation	In PBS, 50% glycerol, pH 7.2.
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Key Applications	Immunoblotting (Western Blotting) Immunoprecipitation
Application Notes	Immunoblotting (1:1000, chemiluminescence) Immunoprecipitation (1:100)
Application Comments	Does not cross-react with HO-2. Variables associated with assay conditions will dictate the proper working dilution.

Biological Information
Immunogen	a synthetic peptide (MERPQPDSMPQDLSEALKEATKEVHTQAEN) corresponding to amino acids 1-30 of human HO-1 prepared as a four-branched multiple antigen peptide
Immunogen	Human
Host	Rabbit
Isotype	IgG
Species Reactivity	Canine Hamster Human Monkey Mouse Rat
Antibody Type	Polyclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information
S Phrase	S: 24/25-36-A09 Avoid contact with skin and eyes. Wear suitable protective clothing.

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
374090-100ULCN	04055977191172

Documentation

Anti-Heme Oxygenase-1 (1-30) Rabbit pAb MSDS

职位
物料安全数据表 (MSDS)

Anti-Heme Oxygenase-1 (1-30) Rabbit pAb 分析证书

标题	批号
374090	输入批号

参考

参考信息概述
Maines, M.D. 1988 FASEB J. 2, 2557. Kutty, R.K., et al. 1994. J. Cell Physiol. 159, 371. Yoshida, T., et al. 1988. Eur. J. Biochem. 171, 457. Trakshel, G.M., et al. 1986 J. Biol. Chem. 261, 11131.

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	15-December-2008 JSW
Synonyms	Anti-HO-1, Anti-Hsp32
Application	Immunoblotting (1:1000, chemiluminescence) Immunoprecipitation (1:100)
Application Data	Detection of heme oxygenase-1 by immunoblotting. Samples: Recombinant rat HO-1 (lane 1), recombinant human HO-1 (lane 2), negative control recombinant human protein (lane 3), extracts from dog liver microsomes (lane 4), mouse liver microsomes (lane 5), rat liver microsomes (lane 6), and human liver microsomes (lane 7). Primary antibody: Anti-Heme Oxygenase-1 (1-30) Rabbit pAb (Cat. No. 374090) (1:1000). Detection: chemiluminescence.
Description	Protein A purified rabbit polyclonal antibody. Recognizes the ~32 kDa HO-1 protein.
Background	Heme oxygenase (HO) is a microsomal enzyme that oxidatively cleaves heme, a pro-oxidant, into carbon monoxide and biliverdin, which is reduced to the antioxidant, bilirubin. Two isozymes, termed HO-1 and HO-2, have been identified. By SDS-PAGE, mammalian HO-1 is ~31-33 kDa; HO-2 is ~36 kDa. HO-1, also referred to as stress protein Hsp32, is the inducible form of the enzyme. HO-1 expression can be induced by a variety of stimuli such as heme, metals, hormones, UV radiation and sulfhydryl depleting agents. In contrast, HO-2 is constitutively expressed. HO-2 is the most prevalent form in most tissues, except the spleen where HO-1 levels are predominant.
Host	Rabbit
Immunogen species	Human
Immunogen	a synthetic peptide (MERPQPDSMPQDLSEALKEATKEVHTQAEN) corresponding to amino acids 1-30 of human HO-1 prepared as a four-branched multiple antigen peptide
Isotype	IgG
Species	canine, hamster, human, monkey, mouse, rat
Form	Liquid
Formulation	In PBS, 50% glycerol, pH 7.2.
Preservative	≤0.1% sodium azide
Comments	Does not cross-react with HO-2. Variables associated with assay conditions will dictate the proper working dilution.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Standard Handling
References	Maines, M.D. 1988 FASEB J. 2, 2557. Kutty, R.K., et al. 1994. J. Cell Physiol. 159, 371. Yoshida, T., et al. 1988. Eur. J. Biochem. 171, 457. Trakshel, G.M., et al. 1986 J. Biol. Chem. 261, 11131.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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