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Recognizes the ~50 kDa HTT protein in U-2 OS and HeLa cells and human salivary gland tissue.
Catalogue Number
ST1529
Brand Family
Calbiochem®
Synonyms
Anti-HD, Anti-Huntingtin
Application Data
Detection of human HTT by immunoblotting. Sample: Whole cell lysate from U-2 OS cells. Primary antibody: Anti-HTT Mouse mAb (3F1) (Cat. No. ST1529) (1-5 µg/ml). Detection: chemiluminescence. Detection of human HTT by immunoblotting. Sample: Whole cell lysate from HeLe NE cells. Primary antibody: Anti-HTT Mouse mAb (3F1) (Cat. No. ST1529) (1-5 µg/ml). Detection: chemiluminescence. Detection of human HTT by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human salivary gland tissue. Primary antibody: Anti-HTT Mouse mAb (3F1) (Cat. No. ST1529) (3 µg/ml). Detection: colorimetric (DAB).
References
Product Information
Form
Liquid
Formulation
In PBS, pH 7.2.
Positive control
U-2 OS cells, HeLa NE cells, Human salivary gland tissue
ELISA (see comments) Immunoblotting (1-5 µg/ml) Paraffin Sections (3 µg/ml)
Application Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
A recombinant polypeptide corresponding to amino acids 81-191 of human HTT, expressed as a GST fusion protein
Clone
3F1
Host
Mouse
Isotype
IgG2a
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
08-November-2010 RFH
Synonyms
Anti-HD, Anti-Huntingtin
Application
ELISA (see comments) Immunoblotting (1-5 µg/ml) Paraffin Sections (3 µg/ml)
Application Data
Detection of human HTT by immunoblotting. Sample: Whole cell lysate from U-2 OS cells. Primary antibody: Anti-HTT Mouse mAb (3F1) (Cat. No. ST1529) (1-5 µg/ml). Detection: chemiluminescence. Detection of human HTT by immunoblotting. Sample: Whole cell lysate from HeLe NE cells. Primary antibody: Anti-HTT Mouse mAb (3F1) (Cat. No. ST1529) (1-5 µg/ml). Detection: chemiluminescence. Detection of human HTT by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human salivary gland tissue. Primary antibody: Anti-HTT Mouse mAb (3F1) (Cat. No. ST1529) (3 µg/ml). Detection: colorimetric (DAB).
Description
Purified mouse monoclonal antibody. Recognizes the ~50 kDa HTT protein.
Background
Huntington's disease is a gene-linked neurodegenerative disorder characterized by loss of striatal neurons. This disorder is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range in the number of trinucleotide repeats has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is ~13.7 kb and is expressed predominantly in adult and fetal brain, whereas the smaller transcript is ~10.3 kb and is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats.
Host
Mouse
Immunogen
A recombinant polypeptide corresponding to amino acids 81-191 of human HTT, expressed as a GST fusion protein
Clone
3F1
Isotype
IgG2a
Species
human
Positive control
U-2 OS cells, HeLa NE cells, Human salivary gland tissue
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
