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MABC675 Sigma-AldrichAnti-HSPA5 Antibody, clone 4E3

Anti-HSPA5 Antibody, clone 4E3 is a highly specific mouse monoclonal antibody, that targets GRP-78 & has been tested in western blotting & IHC.

Anti-HSPA5 Antibody, clone 4E3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: HSPA5, GRP78 UniProt Number: P11021

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, R, M	WB, IHC	M	Ascites	Monoclonal Antibody

Description
Catalogue Number	MABC675
Description	Anti-HSPA5 Antibody, clone 4E3
Alternate Names	78 kDa glucose-regulated protein GRP-78 Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 Heat shock 70 kDa protein 5 Immunoglobulin heavy chain-binding protein BiP
Background Information	The gene HSPA5 encodes a protein named GRP-78 (78 kDa glucose-regulated protein) also known as BiP (immunoglobulin heavy chain-binding protein) or heat shock 70 kDa protein 5. While originally discovered as one of set of genes that were induced upon cellular starvation and stress, HSPA5 encoded GRP-78 is now known to be an important member of the heat-shock family of proteins. The HSPA5 encoded GRP-78 protein appears to play a role in helping in the assembly of multimeric protein complexes in the endoplasmic reticulum (ER). Cellular localization is in the endoplasmic reticulum lumen and appears as cytoplasmic-like staining. The HSPA5 encoded protein is an essential component of the translocation processes within the endoplasmic reticulum and also functions in the retrograde transport across the ER membrane of incorrectly folded proteins destined to be degraded by the ubiquitin proteasome system. The HSPA5 encoded protein, while widely expressed in all cells types under normal conditions, is particularly induced under conditions of cellular stress, heat, or toxins and conditions which lead to the accumulation of unfolded proteins in the ER. EMD-Millipore’s Anti-HSPA5 protein mouse monoclonal antibody, clone 4E3, has been tested by western blot in human HeLa and Jurkat cell lysates as well as mouse NIH3T3 cell lysates. The clone has also been successfully tested in immunohistochemistry in human, paraffin-embedded, thyroid tissues.

References

Product Information
Format	Ascites
Control	HeLa cell lysate
Presentation	Mouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-HSPA5 Antibody, clone 4E3 is a highly specific mouse monoclonal antibody, that targets GRP-78 & has been tested in western blotting & IHC.
Key Applications	Western Blotting Immunohistochemistry
Application Notes	Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected HSPA5 in 10 µg of NIH/3T3, Jurkat, and A431 cell lysates. Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected HSPA5 in rat cerebellum, rat pons/midbrain, rat hippocampus, and human thyroid tissues. Optimal working dilutions must be determined by end user.

Biological Information
Immunogen	Purified recominant fragment of human HSPA5 expressed in E. coli.
Clone	4E3
Host	Mouse
Isotype	IgG1
Species Reactivity	Human Rat Mouse
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_005338
Gene Symbol	HSPA5 GRP78
Purification Method	Unpurified
UniProt Number	P11021
Molecular Weight	~72 kDa observed. Uncharacterized bands may appear in some lysate(s).

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected HSPA5 in 10 µg of HeLa cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
MABC675	04053252910012

Documentation

Anti-HSPA5 Antibody, clone 4E3 MSDS

职位
物料安全数据表 (MSDS)

Anti-HSPA5 Antibody, clone 4E3 分析证书

标题	批号
Anti-HSPA5, clone 4E3 - VP1401281	VP1401281
Anti-HSPA5, clone 4E3 - VP1911082	VP1911082
Anti-HSPA5, clone 4E3 - VP2106031	VP2106031
Anti-HSPA5, clone 4E3 -VP1410288	VP1410288
Anti-HSPA5, clone 4E3 -VP1505224	VP1505224
Anti-HSPA5, clone 4E3 -VP1511120	VP1511120
Anti-HSPA5, clone 4E3 -VP1601278	VP1601278
Anti-HSPA5, clone 4E3 -VP1602240	VP1602240
Anti-HSPA5, clone 4E3 -VP1603021	VP1603021
Anti-HSPA5, clone 4E3 -VP1609039	VP1609039

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描述

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种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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