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Detection of human HPSE by immunoblotting. Sample: Partial recombinant human protein. Primary antibody: Anti-HPSE Mouse mAb (1G12) (Cat. No. ST1611) (1-5 µg/ml). Detection: chemilimunescence.
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
A recombinant polypeptide corresponding to amino acids 72-171 of human HPSE, expressed as a GST fusion protein
Clone
4D7
Host
Mouse
Isotype
IgG2b
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
09-November-2010 RFH
Synonyms
Anti-Heparanase-1
Application
ELISA (see comments) Immunoblotting (1-5 µg/ml)
Application Data
Detection of human HPSE by immunoblotting. Sample: Partial recombinant human protein. Primary antibody: Anti-HPSE Mouse mAb (1G12) (Cat. No. ST1611) (1-5 µg/ml). Detection: chemilimunescence.
Description
Purified mouse monoclonal antibody. Recognizes the HPSE protein.
Background
Heparanases are endo β-D-glucuronidase enzymes that cleave heparan sulfate side chains of heparan sulfate proteoglycans on cell surfaces and the extracellular matrix. Heparanases are involved in the clotting pathway and interact with tissue factor (TF) and TF pathway inhibitor (TFPI) to promote coagulation. Additional physiological roles include heparan sulfate turnover, embryo development, hair growth, and wound healing. Overexpression of heparanases is strongly implicated in tumor metastasis and angiogenesis.
Host
Mouse
Immunogen
A recombinant polypeptide corresponding to amino acids 72-171 of human HPSE, expressed as a GST fusion protein
Clone
4D7
Isotype
IgG2b
Species
human
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
