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MAB3580 Anti-Green Fluorescent Protein Antibody

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MAB3580
100 µL  
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      概述

      Replacement Information

      重要规格表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AELISA, ICC, IHC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB3580
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Green Fluorescent Protein Antibody
      Alternate Names
      • GFP
      • eGFP
      Background InformationThe Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      Control
      • GFP fusion protein expressed in cells
      PresentationLiquid in PBS containing 1 mg/mL BSA. Contains no preservative.
      Quality LevelMQ100
      Applications
      ApplicationDetect Green Fluorescent Protein using this Anti-Green Fluorescent Protein Antibody validated for use in ELISA, IC, IH & WB.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern blot: 1:2,500-1:5,000. Recommended dilution buffer is PBS containing 1 mg/mL BSA.

      Immunocytochemistry: 1:500-1:1,000

      Immunohistochemistry: 1:500-1:1,000 on paraformaldehyde fixed tissues with an overnight incubation using Nickel enhanced DAB for detection. Suggested permeabilization method is 0.5% Triton X-100. Recommended blocking buffer is PBS containing BSA and serum from the host of the secondary antibody. Recommended dilution buffer is PBS containing 1 mg/mL of BSA.

      ELISA: 1:1,000-1:2,000 in a direct assay.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenGFP-BSA
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with Green Fluorescent Protein (GFP). The antibody reacts with various forms of GFP including eGFP including those found in vectors supplied by Clonetech and Invitrogen. There is not cross-reactivity to RFP. YFP has not been tested. This antibody works well for immunohistochemistry.
      IsotypeIgG1
      Species Reactivity
      • All
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular WeightMW for GFP is 27 kDa. Dependent upon the molecular weight of the fusion protein being detected.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      产品目录编号 GTIN
      MAB3580 04053252579530

      Documentation

      Anti-Green Fluorescent Protein Antibody MSDS

      职位

      物料安全数据表 (MSDS) 

      Anti-Green Fluorescent Protein Antibody 分析证书

      标题批号
      MOUSE ANTI-GREEN FLUORESCENT PROTEIN (GFP) MONOCLONAL ANTIBODY - 2118934 2118934
      MOUSE ANTI-GREEN FLUORESCENT PROTEIN (GFP) MONOCLONAL ANTIBODY - 2373312 2373312
      MOUSE ANTI-GREEN FLUORESCENT PROTEIN (GFP) MONOCLONAL ANTIBODY - 2428489 2428489
      MOUSE ANTI-GREEN FLUORESCENT PROTEIN (GFP) - 3207634 3207634
      MOUSE ANTI-GREEN FLUORESCENT PROTEIN (GFP) - 3451414 3451414
      MOUSE ANTI-GREEN FLUORESCENT PROTEIN (GFP) - 3729365 3729365
      MOUSE ANTI-GREEN FLUORESCENT PROTEIN (GFP) - 3852292 3852292
      MOUSE ANTI-GREEN FLUORESCENT PROTEIN (GFP) - 3943774 3943774
      MOUSE ANTI-GREEN FLUORESCENT PROTEIN (GFP) -2519303 2519303
      MOUSE ANTI-GREEN FLUORESCENT PROTEIN (GFP) -2615707 2615707

      参考

      参考概述应用品种公共医疗ID
      shRNA targeting α-synuclein prevents neurodegeneration in a Parkinson's disease model.
      Zharikov, AD; Cannon, JR; Tapias, V; Bai, Q; Horowitz, MP; Shah, V; El Ayadi, A; Hastings, TG; Greenamyre, JT; Burton, EA
      The Journal of clinical investigation  125  2721-35  2015

      显示摘要
      26075822 26075822
      Dystroglycan controls dendritic morphogenesis of hippocampal neurons in vitro.
      Bijata, M; Wlodarczyk, J; Figiel, I
      Frontiers in cellular neuroscience  9  199  2015

      显示摘要
      26074769 26074769
      SNSMIL, a real-time single molecule identification and localization algorithm for super-resolution fluorescence microscopy.
      Tang, Y; Dai, L; Zhang, X; Li, J; Hendriks, J; Fan, X; Gruteser, N; Meisenberg, A; Baumann, A; Katranidis, A; Gensch, T
      Scientific reports  5  11073  2015

      显示摘要
      26098742 26098742
      Nkx2.2 and Nkx2.9 are the key regulators to determine cell fate of branchial and visceral motor neurons in caudal hindbrain.
      Jarrar, W; Dias, JM; Ericson, J; Arnold, HH; Holz, A
      PloS one  10  e0124408  2015

      显示摘要
      25919494 25919494
      Midkine-a protein localization in the developing and adult retina of the zebrafish and its function during photoreceptor regeneration.
      Gramage, E; D'Cruz, T; Taylor, S; Thummel, R; Hitchcock, PF
      PloS one  10  e0121789  2015

      显示摘要
      25803551 25803551
      Polarity-dependent asymmetric distribution and MEX-5/6-mediated translational activation of the Era-1 mRNA in C. elegans embryos.
      Spiró, Z; Gönczy, P
      PloS one  10  e0120984  2015

      显示摘要
      25821955 25821955
      Channel-mediated astrocytic glutamate modulates hippocampal synaptic plasticity by activating postsynaptic NMDA receptors.
      Park, H; Han, KS; Seo, J; Lee, J; Dravid, SM; Woo, J; Chun, H; Cho, S; Bae, JY; An, H; Koh, W; Yoon, BE; Berlinguer-Palmini, R; Mannaioni, G; Traynelis, SF; Bae, YC; Choi, SY; Lee, CJ
      Molecular brain  8  7  2015

      显示摘要
      25645137 25645137
      Erythropoietin produced by genetic-modified NIH/3T3 fibroblasts enhances the survival of degenerating neurons.
      Li, YC; Chen, SJ; Chien, CL
      Brain and behavior  5  e00356  2015

      显示摘要
      26357589 26357589
      Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting.
      Biesemann, C; Grønborg, M; Luquet, E; Wichert, SP; Bernard, V; Bungers, SR; Cooper, B; Varoqueaux, F; Li, L; Byrne, JA; Urlaub, H; Jahn, O; Brose, N; Herzog, E
      The EMBO journal  33  157-70  2014

      显示摘要
      24413018 24413018
      Non-integrating gamma-retroviral vectors as a versatile tool for transient zinc-finger nuclease delivery.
      Bobis-Wozowicz, S; Galla, M; Alzubi, J; Kuehle, J; Baum, C; Schambach, A; Cathomen, T
      Scientific reports  4  4656  2014

      显示摘要
      24722320 24722320

      数据表

      标题
      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

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      种类

      Life Science Research > Antibodies and Assays > Primary Antibodies