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AB5018 Anti-Glutamate (No Glutaraldehyde) Antibody

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AB5018
2000 assays  
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      概述

      重要规格表

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AIHCRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5018
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Glutamate (No Glutaraldehyde) Antibody
      Product Information
      FormatPurified
      Presentation IgG fraction in sterile 0.1M phosphate buffer. No preservative
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Glutamate (No Glutaraldehyde) Antibody is validated for use in IH for the detection of Glutamate (No Glutaraldehyde).
      Key Applications
      • Immunohistochemistry
      Application NotesImmunohistochemistry using silver-intensified immunogold or fluorescence (see recommended protocol). Samples should be fixed with 0.1% - 2.5% glutaraldehyde for optimum detection.

      *This antibody has also been used and found to work with a zero-low glutaraldehyde / high paraformaldehyde fixation (4% paraformaldehyde in 0.1M phosphate buffer / 3% sucrose fixative ). The minimum glutaraldehyde concentration for AB5018 is 0.00%. See protocol that follows. Performance is good with frozen sections, Vibratome sections and tissue culture formats, when penetrating reagents such as 0.3% Triton X-100 are used.

      Optimal working dilutions must be determined by the end user.

      DILUTION: Prepare enough of the AB5018 for your days use by diluting 100X with 1% GSPBT.
      Biological Information
      ImmunogenGlutamate-glutaraldehyde
      HostRabbit
      SpecificityGlutamate (L-Glutamate). The antibody has been calibrated against a spectrum of antigens to assure hapten selectivity and proper affinity. No measurable cross-reactivity (<1:1000) against glutamate in peptides or proteins. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) against L-alanine, gamma-aminobutyrate, 1-amino-4-guanidobutane (AGB), D/L-arganine, D/L-aspartate, L-citrulline, L-cysteine, D/L-glutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine. Modest cross-reactivity (1:20) against D-glutamate. Significant cross-reactivity (1:8) against free NAAG in competition assays (NAAG is not retained in glutaraldehyde-fixed tissue).
      Species Reactivity
      • All
      Antibody TypePolyclonal Antibody
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain stock at 2-8°C in undiluted aliquots for up to 6 months. This stock is extremely stable under normal use and routine storage at 2-8°C. Do not freeze.
      Packaging Information
      Material Size2000 assays
      Global Trade Item Number
      产品目录编号 GTIN
      AB5018 04053252588884