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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

IF03L Sigma-AldrichAnti-Glial Fibrillary Acidic Protein (GFAP) Mouse mAb (G-A-5)

Anti-Glial Fibrillary Acidic Protein, mouse monoclonal, clone G-A-5, recognizes GFAP in astrocytes, astrocytomas, glial cells & gliomas. It is validated for use in WB, IF, IP, IHC (frozen & paraffin).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

分析证书
参考
Data Sheet

同义词: Anti-GFAP

Recommended Products

概述

Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
Ch, H, Porcine, R	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	Recognizes GFAP in astrocytes, astrocytomas, glial cells, gliomas, and other glial cell-derived tumors.
Catalogue Number	IF03L
Brand Family	Calbiochem®
Synonyms	Anti-GFAP

References
References	Droese, M., et al. 1984. Acta. Cytol. 28, 368. Osborn, M., et al. 1984. In: Cancer Cells 1, The Transformed Phenotype. Cold Spring Harbor Lab., 191. Geisler, N. and Weber, K. 1983. EMBO J. 11, 2059. Osborn, M. and Weber, K. 1983. Lab. Invest. 48, 372. Altmannsberger, M., et al. 1982. Lab. Invest. 46, 520. Lazarides, E. 1982. Ann. Rev. Biochem. 51, 219. Osborn, M. and Weber, K. 1982. Cell 31, 303.

References

Droese, M., et al. 1984. Acta. Cytol. 28, 368.
Osborn, M., et al. 1984. In: Cancer Cells 1, The Transformed Phenotype. Cold Spring Harbor Lab., 191.
Geisler, N. and Weber, K. 1983. EMBO J. 11, 2059.
Osborn, M. and Weber, K. 1983. Lab. Invest. 48, 372.
Altmannsberger, M., et al. 1982. Lab. Invest. 46, 520.
Lazarides, E. 1982. Ann. Rev. Biochem. 51, 219.
Osborn, M. and Weber, K. 1982. Cell 31, 303.

Product Information
Form	Lyophilized
Formulation	Lyophilized from 20 mM NH₄HCO₃, 100 µg BSA.
Negative control	Epithelial cells
Positive control	Glial cells (such as U343/333MG)
Preservative	None
Quality Level	MQ100

Applications
Application References	Immunoblotting, Paraffin Sections Sennlaub, F., et al. 2003. Circulation 108, 198. Original Clone Debus, E., et al. 1983. Differentiation 25, 193. Immunofluorescence Debus, E., et al. 1983. Differentiation 25, 193.
Key Applications	Frozen Sections Immunoblotting (Western Blotting) Immunofluorescence Immunoprecipitation Paraffin Sections
Application Notes	Immunoblotting (see application references) Paraffin Sections (see application references) Frozen Sections (see comments) Immunofluorescence (see application references) Immunoprecipitation (see comments)
Application Comments	Stains intermediate filaments in astrocytes, glial cells, gliomas, and other glial cell-derived tumors. This antibody has also been reported to work for frozen sections and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	GFAP
Immunogen	Porcine
Clone	G-A-5
Host	Mouse
Isotype	IgG₁
Species Reactivity	Chicken Human Porcine Rat
Antibody Type	Monoclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Ok to freeze
Special Instructions	Resuspend the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Freezing of aliquots is best for long-term storage of reconstituted product; avoid repetitive freezing and thawing.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
IF03L-100UGCN	04055977228120

Documentation

Anti-Glial Fibrillary Acidic Protein (GFAP) Mouse mAb (G-A-5) 分析证书

标题	批号
IF03L	输入批号

参考

参考信息概述
Droese, M., et al. 1984. Acta. Cytol. 28, 368. Osborn, M., et al. 1984. In: Cancer Cells 1, The Transformed Phenotype. Cold Spring Harbor Lab., 191. Geisler, N. and Weber, K. 1983. EMBO J. 11, 2059. Osborn, M. and Weber, K. 1983. Lab. Invest. 48, 372. Altmannsberger, M., et al. 1982. Lab. Invest. 46, 520. Lazarides, E. 1982. Ann. Rev. Biochem. 51, 219. Osborn, M. and Weber, K. 1982. Cell 31, 303.

参考信息概述

数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	27-August-2007 RFH
Synonyms	Anti-GFAP
Application	Immunoblotting (see application references) Paraffin Sections (see application references) Frozen Sections (see comments) Immunofluorescence (see application references) Immunoprecipitation (see comments)
Description	Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with PAI mouse myeloma cells (see application references). Recognizes the glial fibrillary acidic protein (GFAP).
Background	Intermediate (10 nm) filaments are present in, and provide information concerning the origin of, most vertebrate cells. At present, five major cell types can be distinguished by their intermediate filament components. These include epithelial cells (cytokeratins), neurons (neurofilaments), glial cells (glial fibrillary acidic protein or GFA or GFAP), skeletal, visceral, and certain vascular smooth muscle cells (desmin) and various nonepithelial cells, including cells of mesenchymal origin (vimentin). GFAP and vimentin are coexpressed in at least some astrocytes. Anti-GFAP (Ab-1) identifies the GFAP subclass of intermediate filaments in human tissues.
Host	Mouse
Immunogen species	Porcine
Immunogen	GFAP
Clone	G-A-5
Isotype	IgG₁
Species	chicken, human, porcine, rat
Positive control	Glial cells (such as U343/333MG)
Negative control	Epithelial cells
Form	Lyophilized
Formulation	Lyophilized from 20 mM NH₄HCO₃, 100 µg BSA.
Preservative	None
Comments	Stains intermediate filaments in astrocytes, glial cells, gliomas, and other glial cell-derived tumors. This antibody has also been reported to work for frozen sections and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Ok to freeze
Special Instructions	Resuspend the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Freezing of aliquots is best for long-term storage of reconstituted product; avoid repetitive freezing and thawing.
Toxicity	Standard Handling
References	Droese, M., et al. 1984. Acta. Cytol. 28, 368. Osborn, M., et al. 1984. In: Cancer Cells 1, The Transformed Phenotype. Cold Spring Harbor Lab., 191. Geisler, N. and Weber, K. 1983. EMBO J. 11, 2059. Osborn, M. and Weber, K. 1983. Lab. Invest. 48, 372. Altmannsberger, M., et al. 1982. Lab. Invest. 46, 520. Lazarides, E. 1982. Ann. Rev. Biochem. 51, 219. Osborn, M. and Weber, K. 1982. Cell 31, 303.
Application references	Immunoblotting, Paraffin Sections Sennlaub, F., et al. 2003. Circulation 108, 198. Original Clone Debus, E., et al. 1983. Differentiation 25, 193. Immunofluorescence Debus, E., et al. 1983. Differentiation 25, 193.

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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